Transcriptomic analysis of mouse lungs ofter acute infection with Toxoplasma gondii
CAO Wen-qiao1, LI Kai1, YU Yun1, TAN Qing-qing1, LU He1,2, ZHANG Jing1,2
1.Molecular Medicine and Cancer Research Center of Chongqing Medical University, Chongqing 400016, China; 2.Department of pathogenic biology of basic medicine college Chongqing medical university, Chongqing 400016,China
Abstract：The purpose of this study was to investigate the transcriptomic changes in mouse lungs infected with Toxoplasma gondii. Total RNA was extracted from infected and uninfected mouse lung samples, and a cDNA library was constructed from the mRNA, and then subjected to high throughput RNA sequencing (RNA-seq) with the BGISEQ-500 platform. Ten differentially expressed genes (DEGs) were randomly selected for verification with quantitative real-time PCR (q-PCR). Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and key driver gene analysis (KDA) were performed on the sequencing data. A total of 286 DEGs were identified, among which 234 were up-regulated and 52 were down-regulated. Q-PCR experiments showed consistent trends with the RNA-seq results, indicating that the RNA-seq data were reliable. Most of the GO terms were associated with immunity and cytokines; KEGG enrichment analysis showed that the terms cytokine-cytokine receptor interaction and chemokine signaling pathway were significantly enriched, i.e., Top1 and Top4, respectively. KDA analysis was performed on the 24 DEGs that were significantly enriched in cytokine receptor interaction and chemokine signaling pathway, and ten key driver genes were identified from the 24 DEGs: Tnf, Cxcl10, Il10, Ccl2, Ifng, Cxcl9, Cxcr2, Ccr5, Ccl4, and Ccl7. Collectively, our research revealed ten key driver genes that may play an important role in mouse lungs after acute Toxoplasma gondii infection.
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2021, Vol. 37 
