Abstract:To investigate the genotype and genetic characteristics of hantavirus (HV) in regions of Henan Province, we collected 32 blood specimens from patients with hemorrhagic fever with renal syndrome (HFRS) admitted to the Sixth People's Hospital of Zhengzhou between 2017 and 2018. Hantavirus RNA was reverse transcribed, and regions bearing the S gene sequence and partial M gene sequence were amplified with hemi-nested PCR, sequenced, and manually assembled. Nucleotide sequence similarity among the newly determined sequences along with sequences from other provinces was analyzed, and a phylogenetic tree was constructed with the neighbor-joining method. Of the 32 serum samples, the HV genome fragment was successfully amplified and sequenced in 16 samples, and sequence analysis indicated that all isolates belonged to Hantaan virus (HTNV). The constructed NJ trees based on S and partial M sequences showed similar topological structures. An HN2018/138 strain sharing 91.4%92.1% and 83.2%84.4% similarity with the other 15 strains at the S segment and partial M segment sequences, respectively, formed a single branch. HTNV, the main cause of HFRS in the studied regions of Henan province, showed clear geographic clustering and high genetic variation, possibly due to mutations or imported cases.
马杰, 郑礼钧, 唐静, 李三景, 霍玉奇. 河南省部分区域肾综合征出血热患者汉坦病毒的分子特征[J]. 中国人兽共患病学报, 2021, 37(3): 216-220.
MA Jie, ZHENG Li-jun, TANG Jing, LI San-jing, HUO Yu-qi. Molecular characteristics of hantavirus isolated from patients with hemorrhagic fever with renal syndrome (HFRS) in several regions of Henan Province. Chinese Journal of Zoonoses, 2021, 37(3): 216-220.
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