Single Nucleotide Repeat Sequence Analysis of Bacillus anthracis isolated in Guizhou Province, 2006-2016
MA Qing1, YANG Ping2, LIU Ying1, WANG Yue1, NIE Wei1, YAO Guang-hai1, LI Shi-jun1
1.Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004,China; 2.The People’s Government of Bahuang Town, Bijiang District, Tongren 550081,China;
Abstract：To provide a technical means and scientific basis for the investigation, surveillance, and source tracking of anthrax, single-nucleotide repeat analysis (SNR) was applied to analyze Bacillus anthracis isolated in Guizhou Province from 2006 to 2016. The genomic DNA of Bacillus anthracis isolates from samples from different regions of Guizhou Province was extracted and amplified with PCR primers targeting SNR loci. Then the sequence length of the amplification product of each SNR locus was obtained through capillary electrophoresis. The SNR type was obtained, and the clustering tree and minimum spanning tree based on the length of each SNR locus of the B. anthracis were constructed using Bionumerics 7.6 software. SNR genotyping showed that 71 isolates of Bacillus anthracis were divided into 37 SNR genotypes. Cluster analysis indicated that 71 strains were clustered into groups A and B. Group A, with a total of 27 clades, could be further divided into two subgroups, A1 and A2. Group B, with a total of ten clades, could be divided into subgroups B1 and B2. The minimum tree showed that 71 strains were distributed in seven SNR clone complexes and five singletons. In conclusion, 37 SNR genotypes were identified among the 71 strains of Bacillus anthracis isolated in Guizhou Province from 2006 to 2016, with a diversity of SNR types. The SNR molecular typing method has high resolution for Bacillus anthracis typing. SNR type distribution is correlated with regional and temporal aggregation.
马青, 杨平, 刘英, 王月, 聂炜, 姚光海, 李世军. 2006-2016年贵州省炭疽芽胞杆菌SNR分型分析[J]. 中国人兽共患病学报, 2021, 37(3): 230-235.
MA Qing, YANG Ping, LIU Ying, WANG Yue, NIE Wei, YAO Guang-hai, LI Shi-jun. Single Nucleotide Repeat Sequence Analysis of Bacillus anthracis isolated in Guizhou Province, 2006-2016. Chinese Journal of Zoonoses, 2021, 37(3): 230-235.
2021, Vol. 37 
