猪带绦虫Ts14-3-3.2重组蛋白诱导小鼠免疫应答的研究

doi:10.3969/j.issn.1002-2694.2021.00.037

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摘要目的观察猪带绦虫Ts14-3-3.2重组蛋白在小鼠体内诱导的免疫应答情况。方法将60只雌性昆明小鼠随机分为5组,分别为Ts14-3-3.2重组蛋白50 μg剂量组、100 μg剂量组和150 μg剂量组以及弗氏佐剂对照组和磷酸盐缓冲液(phosphate buffered saline, PBS)对照组,于首次免疫后0 d、28 d、56 d收集血清和脾淋巴细胞,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测血清中特异性IgG及其亚类(IgG1、IgG2a)水平,采用Cell Counting Kit-8(CCK-8)法检测脾淋巴细胞增殖反应,以及通过ELISA法检测脾淋巴细胞培养上清液中IL-2、IFN-γ、IL-4、IL-10水平。结果 Ts14-3-3.2重组蛋白免疫昆明小鼠后,50 μg、100 μg和150 μg剂量组血清特异性IgG、IgG1和IgG2a水平均在首免后28~56 d内升高且56 d均高于28 d,均呈现剂量-效应关系;脾淋巴细胞增殖水平和脾淋巴细胞培养上清液IL-2、IFN-γ、IL-4、IL-10水平在28~56 d升高(IL-10在56 d恢复至正常水平),均在28 d达到峰值,均呈现剂量-效应关系。结论 Ts14-3-3.2重组蛋白可诱导小鼠产生特异性的细胞免疫和体液免疫应答,并呈现剂量-效应关系。

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	刘美辰
	欧阳任辉
	罗波
	周必英

关键词 ：猪带绦虫, Ts14-3-3.2重组蛋白, 小鼠, 免疫应答

Abstract：To observe the immune response induced by Taenia solium Ts14-3-3.2 recombinant protein in mice, we randomly divided 60 female Kunming mice into five groups receiving a 50 μg dose, 100 μg dose or 150 μg dose of Ts14-3-3.2 recombinant protein, Freund’s adjuvant (control group) or PBS (control group). Serum and spleen lymphocytes were collected 0, 28 and 56 days after the first immunization. Specific IgG and its subclasses in serum were detected with an enzyme linked immunosorbent assay (ELISA), the proliferation of spleen lymphocytes was detected with the cell counting kit-8 method, and the levels of IL-2, IFN-γ, IL-4 and IL-10 in the supernatants of spleen lymphocyte cultures were detected with ELISA. The serum specific IgG, IgG1 and IgG2a levels in the 50 μg, 100 μg and 150 μg 14-3-3 recombinant protein dose groups increased 28-56 days after the first immunization and were higher on day 56 than on day 28; all showed a dose-response relationship. The proliferation of spleen lymphocytes and the levels of IL-2, IFN-gamma, IL-4 and IL-10 increased 28-56 days after the first immunization (IL-10 returned to normal levels on day 56) and peaked on day 28; all showed a dose-response relationship. In conclusion, Taenia solium Ts14-3-3.2 recombinant protein induced a specific humoral immune response and cellular immune response in mice, and showed a dose-response relationship.

Key words： Taenia solium 14-3-3.2 recombinant protein mice immune response

收稿日期: 2020-07-10

R383.3

基金资助:贵州省科技计划项目(黔科合基础[2018]1190)、贵州省教育厅青年科技人才成长项目(黔教合[2018]225)和省市科技合作专项资金项目(省市科合[2015]52)联合资助

通讯作者: 周必英,Email: zbyzl01@126.com; ORCID: 0000-0002-5708-4458

引用本文:

刘美辰, 欧阳任辉, 罗波, 周必英. 猪带绦虫Ts14-3-3.2重组蛋白诱导小鼠免疫应答的研究[J]. 中国人兽共患病学报, 2021, 37(4): 330-338. LIU Mei-chen, OUYANG Ren-hui, LUO Bo, ZHOU Bi-ying. Study of the mouse immune response induced by Taenia solium Ts14-3-3.2 recombinant protein. Chinese Journal of Zoonoses, 2021, 37(4): 330-338.

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http://www.rsghb.cn/CN/10.3969/j.issn.1002-2694.2021.00.037 或 http://www.rsghb.cn/CN/Y2021/V37/I4/330

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