S-adenosylmethionine synthase is involved in Mycoplasma pneumoniae biofilm formation
LI Ting-ting1, CHEN Chun-yan1,2, YU Lan1, 3, DING Wei-yan1, DING Nan1, LI Shui-hong1, ZHU Cui-ming1
1. Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, China;
2. Clinical Laboratory of the Central Hospital of Xiangtan,Xiangtan 411100, China;
3. Department of Blood Transfusion, the First Afiliated Hospital of University of South China,Hengyang 421001, China
Abstract：This study aimed to explore the influence of S-adenosylmethionine synthase on Mycoplasma pneumoniae biofilm production, to better understand the underlying mechanisms. M. pneumoniae Mp129 strains and 22 clinical isolates were cultured in 24-well plates. After crystal violet staining, M. pneumoniae biofilms were observed under a light microscope and semi-quantitatively analyzed according to the OD value at 570 nm. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression levels of the S-adenosylmethionine synthase gene (metX) in three M. pneumoniae strains with biofilm formation and three strains with weak or no biofilm formation. The effects of sinefungin and rosmarinic acid on the metX mRNA transcription level and biofilm formation in the M. pneumoniae strains was evaluated in vitro. Among the 23 M. pneumoniae strains, 13 strains formed biofilms, whereas 10 isolates did not form biofilms or produced only a small amount of biofilms in 24-well plates. The mRNA expression level of metX was much higher in the M. pneumoniae strains with biofilm formation than in those with weak or no biofilm formation (P<0.05). Furthermore, the metX transcription level and mycoplasmal biofilm formation were significantly inhibited by sinefungin and increased by rosmarinic acid. The formation of M. pneumoniae biofilm correlated with the metX mRNA transcription level; therefore, S-adenosylmethionine synthase may be involved in the formation of M. pneumoniae biofilm.
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