Effects of Hymenolepis nana infection on the expression of LY6A (Sca-1), IFN-γ and STAT1 in the small intestine in ICR mice
ZHAO Jing1,2, YANG Han-lei1,2, CHEN Su-su1,2, MOU Rong1,2
1. Department of Parasitology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China; 2. Characteristic and Key Laboratory of Modern Pathogenic Biology, Guizhou Medical University, Guiyang 550025, China
Abstract:This study aimed to investigate the expression of LY6A, IFN-γ and STAT1 in the small intestine in ICR mice infected with Hymenolepis nana. Adult specimens of Hymenolepis nana were collected, and the eggs were retrieved and used to make a suspension. ICR mice were randomly divided into a control group and experimental group. The experimental group was infected with 1000 eggs per mouse. On the 2nd and 8th days after infection, the mice were killed according to the number, and small intestinal tissue was obtained. The histopathology of the small intestine was observed with HE staining, the relative expression of LY6A, IFN-γ and STAT1 mRNA was detected by RT-PCR, the expression of LY6A protein positive cells in the small intestine was detected by immunohistochemistry, and the relative abundance of LY6A and STAT1 proteins was quantified with the PRM technique. The results of HE staining showed that adult segments were found in the intestinal cavity on the 8th day after infection, and an acute inflammatory reaction was observed in the parasitic part of the parasite. RT-PCR detection showed that the relative expression of LY6A (t=12.57, P<0.001) and STAT1 (t=12.13, P<0.001) mRNA on the second day of infection in the experimental group was lower than that in the control group, and the relative expression of IFN-γ mRNA in the experimental group was higher than that in the control group (t=7.78,P<0.001). On the 8th day after infection, the relative expression of LY6A (t=10.01, P<0.001) and STAT1(t=11.19, P<0.001) mRNA in the experimental group was higher than that in the control group (t=26.47, P<0.001), and the relative expression of IFN-γ mRNA was lower than that in the control group. The immunohistochemistry results showed that the percentage of LY6A positive cells in the experimental group was higher than that in the control group on the second day after infection (t=4.26,P<0.01), and the percentage of LY6A protein positive cells in the experimental group was significantly higher than that in the control group on the 8th day after infection (t=8.18, P<0.001). The results of PRM detection showed that the relative expression of LY6A protein in the experimental group was lower than that in the control group on the second day after infection (t=6.55,P<0.05), but there was no significant difference between the experimental group and the control group. On the 8th day after infection, the relative expression of LY6A (t=4.95, P<0.05) and STAT1 (t=2.91, P<0.05)proteins in the experimental group was significantly higher than that in the control group.The mRNA and protein levels of LY6A (Sca-1) in the small intestine in ICR mice infected with Hymenolepis nana showed low expression in early stages of larval invasion and high expression in the adult stage, IFN-γ did not play a major role in the expression of LY6A, whereas STAT1 may induce the expression of LY6A (Sca-1).
赵敬, 杨汉蕾, 陈素素, 牟荣. 微小膜壳绦虫感染对ICR小鼠小肠LY6A(Sca-1)及IFN-γ、STAT1表达的影响[J]. 中国人兽共患病学报, 2021, 37(7): 611-617.
ZHAO Jing, YANG Han-lei, CHEN Su-su, MOU Rong. Effects of Hymenolepis nana infection on the expression of LY6A (Sca-1), IFN-γ and STAT1 in the small intestine in ICR mice. Chinese Journal of Zoonoses, 2021, 37(7): 611-617.
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