Genotyping by MLVA reveals the regional distribution of Yersinia pestis in Gansu Province
GUO Li-min1, XI Jin-xiao1, GE Ya-jun2, WANG Yu-meng3, MIAO Ke-jun1, WU Bin1, XU Da-qin1
1. Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730020, China; 2. Shanghai Public Health Clinical Center, Fudan University, Shanghai 201058, China; 3. Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：Multiple variable number tandem repeat (VNTR) analysis (MLVA) was used to study the genotypes and distribution of Yersinia pestis in Gansu province. Different ecotypes of Yersinia pestis from 1962 to 2014 were selected for genotyping by MLVA. PCR amplification was carried out at the 14 + 12 VNTR loci, and the amplicons were analyzed by capillary electrophoresis to determine the repeat number of each VNTR locus. The data were analyzed with BioNumerics 5.10. The strains of Y. pestis in Gansu province were divided into ten genotypes. The strains from different foci clustered into different types, which correlated well with the isolation sites. According to the isolation sites, the strains in the types were further subdivided into 5-28 subbranches. The ten genotypes included strains from the Spermophilus alaschanicus plague focus in one type; those from the Gannan Plateau plague focus in one type; those from Akesai County in two types; i.e., those from Dangjinshan and Jiaerwuzong village; those from Subei County in three types; i.e., the types from Machang, Dangchengwan Town and Yuerhong; those from Yumen City, which shared the same type as those from Yuerhong in Subei County; and those from Sunan County in three types; i.e., types from Dahe Town, Mati Town and Kangle Town. The MLVA (14 + 12) scheme can be used to distinguish strains from different plague foci in Gansu province, and the genotypes correlate well with the plague bacteria isolation sites. This method can be used for source tracing of Yersinia pestis.
郭丽民, 席进孝, 葛亚俊, 王宇萌, 苗克军, 吴斌, 徐大琴. 甘肃省鼠疫耶尔森菌多位点可变数目串联重复序列基因分型及地区分布[J]. 中国人兽共患病学报, 2021, 37(8): 715-720.
GUO Li-min, XI Jin-xiao, GE Ya-jun, WANG Yu-meng, MIAO Ke-jun, WU Bin, XU Da-qin. Genotyping by MLVA reveals the regional distribution of Yersinia pestis in Gansu Province. Chinese Journal of Zoonoses, 2021, 37(8): 715-720.
2021, Vol. 37 
