Abstract：This study determined the drug sensitivity of Salmonella in active surveillance of food-borne diseases in Shanxi Province and examined the gene and molecular typing of quinolone-resistant Salmonella to provide a scientific basis for the clinical treatment of Salmonella and a preliminary understanding of the Salmonella drug resistance mechanism. Salmonella isolated from active surveillance cases of food-borne disease in Shanxi Province from 2014 to 2017 were analyzed by serological typing, drug sensitivity tests, quinolone resistance gene detection and pulsed field gel electrophoresis molecular typing. A total of 137 Salmonella strains were classified into 16 serotypes, including 51 strains of Salmonella enteritidis, 47 strains of Salmonella typhimurium and 39 strains of other Salmonella. The resistance rate of 137 strains of Salmonella to nalidixic acid was the highest (56.93%) and was followed by tetracycline (34.31%). Lower resistance rates were found to cefotaxime (8.03%), gentamicin (5.84%) and cefoxitin (0.73%). The gyrA and parC gene point mutations were detected in 78 Salmonella strains resistant to nalidixic acid, and 129 gyrA and parC gene point mutations were found. The similarity of 51 strains of Salmonella enteritidis ranged from 70.6% to 100%, and that of 21 strains of Salmonella typhimurium ranged from 58.9% to 100%. Salmonella enteritidis and Salmonella typhimurium were the main serotypes in Shanxi Province. The drug resistance of Salmonella to nalidixic acid was most severe in this area. More attention should be paid to the surveillance of the drug resistance of Salmonella and the study of the mechanism of quinolone resistance.
姚素霞, 郝瑞娥, 王洋, 张秋香, 杨红霞, 韩吉婷, 秦文彦. 2014-2017年山西省沙门氏菌分子分型及耐药性研究[J]. 中国人兽共患病学报, 2021, 37(9): 815-820.
YAO Su-xia, HAO Rui-e, WANG Yang, ZHANG Qiu-xiang, YANG Hong-xia, HAN Ji-ting, QIN Wen-yan. Analysis of antimicrobial susceptibility and molecular typing of Salmonella in Shanxi Province during 2014-2017. Chinese Journal of Zoonoses, 2021, 37(9): 815-820.
2021, Vol. 37 
