MLVA genotyping of Yersinia pestis in Shaanxi Province
NIE Shou-min1, LUO Bo-yan1, SUN Yang-xin1, FAN Suo-ping1, CHANG Wen-hui1, WANG Yu-meng2, SUN Jie3, AN Cui-hong1
1. Center for Disease Control and Prevention of Shaanxi Province,Xi’an 710054,China; 2. Infectious Disease Control Institute of Chinese Center for Disease Control and Prevention, Beijing 102206, China; 3. Center for Disease Control and Prevention of Chengcheng, Weinan 715200, China
Abstract:This study aimed to study the MLVA genotyping characteristics of Yersinia pestis isolates in Shaanxi Province and to elucidate the genetic evolutionary relationships among strains in different epidemic years, to provide a scientific basis for future plague monitoring and prevention. Genotyping of 66 strains of Y.pestis isolated from plague foci in Shaanxi Province was conducted by MLVA(14+12) in Bionumerics (version 7.6) software to analyze the typing results. Among the 26 loci selected by MLVA, 12 loci (MS56, M15, N2117, N2557, N3773, M33, M34, M22, M43, M23, M25 and M28) were of significance for studying the genetic polymorphism of Y. pestis in Shaanxi Province. A total of 66 Y. pestis strains were divided into 3 groups and 19 genotypes. Genotypes of Y. pestis in Shaanxi Province were found to be polymorphic by MLVA, and genotypes were found to differ among epidemic years. MLVA has great prospects for applications in studying the evolution of Y.pestis and tracing epidemic outbreaks.
聂守民, 罗波艳, 孙养信, 范锁平, 常文辉, 王宇萌, 孙杰, 安翠红. 陕西省鼠疫耶尔森菌MLVA分型研究[J]. 中国人兽共患病学报, 2021, 37(10): 878-884.
NIE Shou-min, LUO Bo-yan, SUN Yang-xin, FAN Suo-ping, CHANG Wen-hui, WANG Yu-meng, SUN Jie, AN Cui-hong. MLVA genotyping of Yersinia pestis in Shaanxi Province. Chinese Journal of Zoonoses, 2021, 37(10): 878-884.
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