Abstract：This study aimed to investigate the serotype distribution, molecular types and drug resistance of Salmonella by analyzing the etiology of Salmonella isolated from patients with diarrhea in the active surveillance sentinel hospital in Jinan from 2017 to 2020. A total of 90 strains of Salmonella isolated from patients with diarrhea from 2017 to 2020 were analyzed by pulsed-field gel electrophoresis (PFGE), and the similarities among strains was analyzed with BioNumerics software. A total of 40 strains of Salmonella randomly selected from the 90 strains were analyzed with drug susceptibility tests with the micro-broth dilution method. The results showed that 27 serotypes among the isolated Salmonella. Salmonella typhimurium and Salmonella enteritidis were dominant, accounting for 47.78% and 17.78% of the total, respectively. PFGE analysis showed that the bands for Salmonella with the same serotype were similar, e.g., Salmonella typhimurium and Salmonella enteritidis, whereas those of different serotypes were polymorphic. The tested strains had a maximal rate of resistance to ampicillin of 72.50% and a multi-drug resistance rate of 67.50%. In conclusion, Salmonella in the Jinan Area shows serotype and genetic diversity, high drug resistance and severe multi-drug resistance. Therefore, drug resistance surveillance in patients and the environment should be intensified.
李娜, 刘辉, 李健, 刘铭, 姜慧钰, 陈潇, 关恒云, 刘岚铮, 张京云. 济南市腹泻患者沙门氏菌PFGE分子分型及耐药特征研究[J]. 中国人兽共患病学报, 2021, 37(10): 893-897.
LI Na, LIU Hui, LI Jian, LIU Ming, JIANG Hui-yu, CHEN Xiao, GUAN Heng-yun, LIU Lan-zheng, ZHANG Jing-yun. PFGE molecular typing and antimicrobial resistance testing of Salmonella from patients with diarrhea in Jinan, China. Chinese Journal of Zoonoses, 2021, 37(10): 893-897.
2021, Vol. 37 
