Comparison of fibrosis in the surrounding tissues of hepatic echinococcosis granulosus and echinococcosis multilocularis in mice at different stages
XING Zhi-kun, WANG Er-qiang, LIAO Yuan, GAO Feng-yi, ZHAO Xue-yuan, TAN Xiao-wu, YU Xiao-fan, WU Xiang-wei
1. School of Public Health for Weifang Medical University, Weifang 261053, China; 2. Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China; 3. School of Management for Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Abstract：In this study, the fibrosis of tissues around the lesions of two types of hepatic echinococcosis in different time periods was compared to increase understanding of the pathophysiological development of hepatic echinococcosis, and to provide information to aid the diagnosis and treatment of hepatic echinococcosis. The animal model was established, and HE, Masson and immunohistochemical staining of COL1, COL3, α-SMA and TGF-β1 were used to compare and observe the differences in fibrosis in the two kinds of hepatic echinococcosis at different times. The results showed that the fibrosis around the hepatic Echinococcus granulosus lesions changed from diffuse to aggregated with time, and could form a continuous and dense outer fibrous membrane. The fibrosis around the hepatic Echinococcus multilocularis lesions was always diffuse, and no continuous and dense outer fibrous membrane could be observed. The intensity of expression of COL1(r=-0.768,P<0.05)、COL3(r=-0.781,P<0.05)、α-SMA(r=-0.867,P<0.05)、TGF-β1(r=-0.854,P<0.05) around the lesions in the E. granulosus group was negatively correlated with time, while the intensity of expression of(r=-0.349,P>0.05)、COL3(r=-0.037,P>0.05)、α-SMA(r=-0.107,P>0.05)、TGF-β1(r=-0.148,P>0.05) around the lesions in the multilocular echinococcosis group was not correlated with time . The collagen content of the extracellular matrix around the two types of hydatid was different. The collagen type I and III ratio of the E. granulosus group was higher than that of the E. multilocularis group(Z=-3.23,P<0.05). Compared with multilocular echinococcosis, continuous and dense fibrous outer sacs can be formed around the lesions of E. granulosus. After the formation of the outer cyst, the process of fibrosis was weakened or stopped. The cases of multilocular echinococcosis showed active fibrosis throughout the course of the disease. E. granulosus has a higher type I/III collagen ratio than the outer capsule of E. multilocularis.
邢稚坤, 王二强, 廖原, 高风亦, 赵雪源, 谭小武, 俞晓凡, 吴向未. 小鼠肝细粒棘球蚴和多房棘球蚴病不同时期病灶周围组织纤维化对比[J]. 中国人兽共患病学报, 2021, 37(11): 1008-1016.
XING Zhi-kun, WANG Er-qiang, LIAO Yuan, GAO Feng-yi, ZHAO Xue-yuan,TAN Xiao-wu, YU Xiao-fan, WU Xiang-wei. Comparison of fibrosis in the surrounding tissues of hepatic echinococcosis granulosus and echinococcosis multilocularis in mice at different stages. Chinese Journal of Zoonoses, 2021, 37(11): 1008-1016.
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