Abstract：To analyze the molecular characteristics and trace the origin of dengue virus in Hangzhou, 2019. Serum samples and epidemiological characteristics were collected from dengue fever cases. Dengue antibodies tests, virus detecting and serotyping were performed by ELISA and RT-PCR, respectively. Phylogenetic trees based on E gene sequence of the dengue virus were then constructed and analyzed. A total of 212 positive samples (158 imported cases and 54 indigenous cases) were collected, among which 65 were positive for IgM, and 178 were positive for DENV RNA. All four serotypes of DENV were detected in imported cases, with a predominance of DENV-1 viruses, accounting for 51.9% of the cases. The indigenous DENV viruses belonged to DENV-1 genotype I, DENV-1 genotype IV, and DENV-2 Cosmopolitan genotype, and were respectively closely related to the strains from Guangzhou and Nanchang in 2019, Philippines in 2015, and Thailand in 2018. Indigenous DENV-1 genotype IV viruses had a high level of E gene sequence similarity (99.9%~100% homology), and their transmission may be caused by multiple imported cases. Affected by the massive dengue epidemic in Southeast Asian countries, an obvious increase in dengue fever cases was observed in Hangzhou in 2019, accompanied with diversifying serotypes detected and more complicated analysis in tracing origin. Further researches should be conducted to analyze the molecular characteristics of DENV.
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