Isolation and identification of mammalian orthoreovirus (YNSZ/V207/2017) from bovine blood samples in Yunnan
LI Zhan-hong1, XIAO Lei1, LI Zhuo-ran1, SONG Zi-ang2, XIE Jia-rui1, YANG Zhen-xing1, ZHU Pei1, LI Hua-chun1, LIAO De-fang1, YANG Heng1,3
1. Yunnan Tropical and Subtropical Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, China; 2. College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China; 3. College of Agriculture and Life Sciences, Kunming University, Kunming 650214, China
Abstract:This study investigated the diversity of viruses and related epidemics in animals in Yunnan Province. Blood samples were collected weekly from ten sentinel cattle in Shizong County, and cells were inoculated for viral isolation. The identity and prevalence of the isolated viruses were confirmed through RT-PCR, electron microscopy observation, whole genome sequencing and serum neutralization testing. Two strains of bluetongue virus, three strains of palyam virus, two strains of epidemic hemorrhagic disease virus and one unidentified virus (YNSZ/V207/2017) were isolated from the blood samples of sentinel cattle. The virion of YNSZ/V207/2017 was approximately 80 nm in diameter and showed icosahedral symmetry under electron microscopy. Whole genome sequence analysis indicated that YNSZ/V207/2017 belonged to mammalian orthoreovirus serotype 1 (MRV-1) and shared the highest sequence similarity with the corresponding segments of MRV isolated from humans, Odocoileus virginianus, Mustla vison, Paguma larvata taivana and Eptesicus serotinus. Serum neutralization tests indicated that the positivity rates of MRV-1 antibodies in local cattle, sheep and pigs were 32.5%, 20.0% and 42.5%, respectively. In conclusion, an MRV-1 strain isolated from cattle in Yunnan Province may be a gene reassortant strain from different host sources, and the virus was found to be widespread in local livestock.
李占鸿, 肖雷, 李卓然, 宋子昂, 谢佳芮, 杨振兴, 朱沛, 李华春, 廖德芳, 杨恒. 云南牛血液标本中哺乳动物正呼肠孤病毒(YNSZ/V207/2017)的分离鉴定[J]. 中国人兽共患病学报, 2022, 38(1): 1-9.
LI Zhan-hong, XIAO Lei, LI Zhuo-ran, SONG Zi-ang, XIE Jia-rui, YANG Zhen-xing, ZHU Pei, LI Hua-chun, LIAO De-fang, YANG Heng. Isolation and identification of mammalian orthoreovirus (YNSZ/V207/2017) from bovine blood samples in Yunnan. Chinese Journal of Zoonoses, 2022, 38(1): 1-9.
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