Study of the temperature sensitivity of severe fever with thrombocytopenia syndrome virus
WEI Xue-min1, LI Shu-han1, WANG Yao1, CHEN Meng-ting1, ZHAO Li1, WANG Zhi-yu1, WAN Min2, WEN Hong-ling1
1. Department of Microbiological Laboratory Technology, School of Public Health, Cheeloo College of Medicine, Shandong University; Key Laboratory of Infectious Disease Control and Prevention in Universities of Shandong, Jinan 250012, China; 2. Shandong Institute of Medical Device and Pharmaceutical Packaging Inspection, Jinan 250101, China
Abstract:This study investigated the temperature sensitivity of severe fever with thrombocytopenia syndrome virus (SFTSV) to provide a basis for SFTSV disinfection and laboratory biosafety protection. We divided SFTSV cell culture supernatants into 250 μL PCR vials at 100 μL/tube, and placed them in a refrigerator at 4 ℃, and a metal bath at 25 ℃, 37 ℃, 39 ℃, 56 ℃, and 70 ℃. After treatment for predetermined periods of time, the viral titer was determined through indirect immunofluorescence in Vero cells. With increasing temperature, the rate of decline of the viral titer increased. After incubation at 4 ℃, 25 ℃, 37 ℃, and 39 ℃ for 24 h, the titers decreased from 107.25/100 μL to 107.00/100 μL, 106.75/100 μL, 106.50/100 μL, and 105.00/100 μL, respectively. At the same temperature, with prolonged storage time, the decrease in titer became more pronounced. After SFTSV was placed at 4 ℃, 25 ℃, 37 ℃ for 72 h, the viral titer decreased from 107.25/100 μL to 106.63/100 μL, 106.50/100 μL, and 103.38/100 μL, respectively. SFTSV lost its infectivity after incubation at 39 ℃ for 72 h. SFTSV was inactivated after exposure to 56 ℃ for 180 min or 70 ℃ for 5 min. We concluded that SFTSV is inactivated after incubation at 70 ℃ for 5 min. However, after 3 days of exposure to 4 ℃ and 25 ℃, the viral titer did not change significantly. Laboratories and medical staff should focus on personal protection and disinfection of items contaminated by SFTSV.
韦雪敏, 李书晗, 王耀, 陈梦婷, 赵丽, 王志玉, 万敏, 温红玲. 发热伴血小板减少综合征病毒温度敏感性研究[J]. 中国人兽共患病学报, 2022, 38(1): 25-28.
WEI Xue-min, LI Shu-han, WANG Yao, CHEN Meng-ting, ZHAO Li, WANG Zhi-yu, WAN Min, WEN Hong-ling. Study of the temperature sensitivity of severe fever with thrombocytopenia syndrome virus. Chinese Journal of Zoonoses, 2022, 38(1): 25-28.
[1] 董利昭. 发热伴血小板减少综合征临床特点及死亡因素分析[D]. 南京:南京医科大学,2018. [2] 苗东. 发热伴血小板减少综合征动态分布及传播风险预测研究[D]. 北京:军事科学院,2020. [3] 任祥春,刘本付,王枫,等. 一起发热伴血小板减少综合征聚集性疫情特征[J]. 国际流行病学传染病学杂志,2021,48(2):150-152. [4] Tsuru M, Suzuki T, Murakami T, et al.Pathological Characteristics of a Patient with Severe Fever with Thrombocytopenia Syndrome (SFTS) Infected with SFTS Virus through a Sick Cat’s Bite[J]. Viruses,2021,13(2):204. DOI: 10.3390/v13020204 [5] Kirino Y, Ishijima K, Miura M, et al.Seroprevalence of severe fever with thrombocytopenia syndrome virus in small-animal veterinarians and nurses in the Japanese Prefecture with the highest case load[J]. Viruses,2021,13(2):229. DOI: 10.3390/v13020229 [6] Kida K, Matsuoka Y, Shimoda T, et al.A case of cat-to-human transmission of severe fever with thrombocytopenia syndrome virus[J]. Jpn J Infect Dis,2019,72(5):356-358. DOI: 10.7883/yoken.JJID.2018.526 [7] 刘建伟. 发热伴血小板减少综合征病毒传播机制及人源单克隆抗体研究[D].济南: 山东大学,2017. [8] 张全福,李建东,姜晓林,等. 发热伴血小板减少综合征病毒热稳定性和灭活条件初步研究[J]. 中华实验和临床病毒学杂志,2014,28(3):206-208. [9] Jung I Y, Choi W, Kim J, et al.Nosocomial person-to-person transmission of severe fever with thrombocytopenia syndrome[J]. Clin Microbiol Infect,2019,25(5):631-633. DOI: 10.1016/j.cmi.2019.01.006 [10] Yu K, Jeong H, Park S, et al. Shedding and transmission modes of severe fever with thrombocytopenia syndrome phlebovirus in a ferret model[J]. Open Forum Infect,2019,6(8):ofz309. DOI: 10.1093/ofid/ofz309 [11] Moon J, Lee H, Jeon JH, et al.Aerosol transmission of severe fever with thrombocytopenia syndrome virus during resuscitation[J]. Infect Control Hosp Epidemiol,2019,40(2):238-241. DOI: 10.1017/ice.2018.330 [12] Kim WY, Choi W, Park SW, et al.Nosocomial transmission of severe fever with thrombocytopenia syndrome in Korea[J]. Clin Infect Dis,2015,60(11):1681-1683. DOI: 10.1093/cid/civ128 [13] 李婕,严冬梅,张勇,等. 肠道病毒A组71型热力和常见3种化学消毒剂灭活效果的研究[J]. 中华实验和临床病毒学杂志,2020,34(1):18-19. DOI:10.3760/cma.j.issn.1003-9279.2020.01.004