Advances in methods for the detection of animal coronaviruses
HE Xun1,2, YOU Lan1,2, FAN Zi-wei1,2, ZHENG Tian-li1,2, CHEN Jia-yi1,2, PEI Xiao-fang1,2
1. West China School of Public Health (West China Fourth Hospital), Sichuan University, Chengdu 610041,China; 2. Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu 610041,China;
Abstract：Since the end of December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed a serious threat to global public health security. Many coronaviruses, including SARS-CoV-2, are of animal origin. Therefore, monitoring of animal coronavirus must be strengthened. Herein, the common sample types, cell types used for viral isolation and culture, viral molecular detection methods, and immunological detection of animal coronaviruses are reviewed to provide a reference for follow-up studies of animal coronaviruses.
何迅, 游兰, 范紫玮, 郑田利, 陈嘉熠, 裴晓方. 动物冠状病毒检测方法的研究进展[J]. 中国人兽共患病学报, 2022, 38(1): 42-47.
HE Xun, YOU Lan, FAN Zi-wei, ZHENG Tian-li, CHEN Jia-yi, PEI Xiao-fang. Advances in methods for the detection of animal coronaviruses. Chinese Journal of Zoonoses, 2022, 38(1): 42-47.
[1] Zhou P, Yang XL, Wang XG, et al.A pneumonia outbreak associated with a new coronavirus of probable bat origin[J]. Nature, 2020,579(7798):270-273. DOI:10.1038/s41586-020-2012-7 [2] Cui J, Li F, Shi ZL.Origin and evolution of pathogenic coronaviruses[J]. Nat Rev Microbiol, 2019,17(3):181-192. DOI:10.1038/s41579-018-0118-9 [3] Chen S, Liu D, Tian J, et al.Molecular characterization of HLJ-073, a recombinant canine coronavirus strain from China with an ORF3abc deletion[J]. Arch Virol, 2019,164(8):2159-2164. DOI:10.1007/s00705-019-04296-9 [4] Lednicky JA, Tagliamonte MS, White SK, et al. Emergence of porcine delta-coronavirus pathogenic infections among children in Haiti through independent zoonoses and convergent evolution[J/OL]. Medrxiv,(2021-03-25)[2021-03-28]. https://www.medrxiv.org/content/10.1101/2021.03.19.21253391v1 [5] Chen D,Sun J,Zhu J,et al.Single-cell screening of SARS-CoV-2 target cells in pets, livestock, poultry and wildlife[J/OL].Biorxiv,(2020-06-14)[2021-03-28].https://www.biorxiv.org/content/10.1101/2020.06.13.149690v1 [6] 贾燕, 关平原. 209例宠物犬冠状病毒病流行病学调查[J]. 畜牧与饲料科学, 2017,38(4):97-100. DOI:10.3969/j.issn.1672-5190.2017.04.035 [7] 吴晓平, 潘书磊, 周五朵, 等. 番鸭源传染性支气管炎病毒PTFY株的分离鉴定[J]. 病毒学报, 2016,32(2):203-209. [8] Hasoksuz M, Hoet AE, Loerch SC, et al.Detection of respiratory and enteric shedding of bovine coronaviruses in cattle in an Ohio feedlot[J]. J Vet Diagn Invest, 2002,14(4):308-313. DOI:10.1177/104063870201400406 [9] Vitosh-Sillman S, Loy JD, Brodersen B, et al.Experimental infection of conventional nursing pigs and their dams with Porcine deltacoronavirus[J]. J Vet Diagn Invest, 2016,28(5):486-497. DOI:10.1177/1040638716654200 [10] Zhang J.Porcine deltacoronavirus: overview of infection dynamics, diagnostic methods, prevalence and genetic evolution[J]. Virus Res, 2016,226:71-84. DOI:10.1016/j.virusres.2016.05.028 [11] 刘振格, 侯喜林, 余丽芸, 等. 牛冠状病毒DQ株感染BALB/c小鼠的敏感性研究[J]. 中国预防兽医学报, 2014,36(1):15-18. DOI:10.3969/j.issn.1008-0589.2014.01.04 [12] Hasoksuz M, Hoet AE, Loerch SC, et al.Detection of respiratory and enteric shedding of bovine coronaviruses in cattle in an Ohio feedlot[J]. J Vet Diagn Invest, 2002,14(4):308-313. DOI:10.1177/104063870201400406 [13] 董志华, 范文胜, 赵长润, 等. 2009-2017年广西地区鸡传染性支气管炎流行病学调查[J]. 中国兽医学报, 2019,39(9):1691-1696. [14] 张昕, 黄坚, 张萍, 等. 成都地区宠物犬感染犬瘟热病毒和犬呼吸道冠状病毒的分子流行病学调查[J]. 中国畜牧兽医, 2018,45(2):486-492. DOI:10.16431/j.cnki.1671-7236.2018.02.025 [15] 俞向前, 艾旻, 张忠海, 等. 犬细小病毒与犬冠状病毒双重PCR检测方法的建立[J]. 上海畜牧兽医通讯, 2017,4:10-12. [16] Guan X, Li H, Han M, et al.Epidemiological investigation of feline infectious peritonitis in cats living in Harbin, Northeast China from 2017 to 2019 using a combination of an EvaGreen-based real-time RT-PCR and serum chemistry assays[J]. Mol Cell Probes, 2020,49:101495. DOI:10.1016/j.mcp.2019.101495 [17] Hu H, Jung K, Vlasova AN, et al.Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States[J]. J Clin Microbiol, 2015,53(5):1537-1548. DOI:10.1128/jcm.00031-15 [18] 沈美艳, 孙秋艳, 李舫, 等. 上海株犬冠状病毒的分离鉴定及S基因的遗传进化分析[J]. 中国动物检疫, 2017,34(3):97-101. DOI:10.3969/j.issn.1005-944X.2017.03.025 [19] Bhuiyan ZA, Ali MZ, Moula MM, et al.Prevalence and molecular characterization of infectious bronchitis virus isolated from chicken in Bangladesh[J]. Vet World, 2019,12(6):909-915. DOI:10.14202/vetworld.2019.909-915 [20] Wang C, Yan F, Zheng X, et al.Porcine epidemic diarrhea virus virus-like particles produced in insect cells induce specific immune responses in mice[J]. Virus Genes, 2017,53(4):548-554. DOI:10.1007/s11262-017-1450-2 [21] Jung K, Miyazaki A, Hu H, et al.Susceptibility of porcine IPEC-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PDCoV) and serum cytokine responses of gnotobiotic pigs to acute infection with IPEC-J2 cell culture-passaged PDCoV[J]. Vet Microbiol, 2018,221:49-58. DOI:10.1016/j.vetmic.2018.05.019 [22] 何琪富, 汤承, 郭紫晶, 等. 牦牛源牛冠状病毒部分基因的扩增、序列分析及病毒分离鉴定[J]. 畜牧兽医学报, 2019,50(2):343-353. DOI:10.11843/j.issn.0366-6964.2019.02.012 [23] Terada Y, Kuroda Y, Morikawa S, et al.Establishment of a virulent full-length cDNA clone for type I feline coronavirus strain C3663[J]. J Virol, 2019,93(21):e01208-19. DOI:10.1128/JVI.01208-19 [24] 唐小娟. 武汉市猫传染性腹膜炎病例分析及病毒的分离与鉴定[D]. 武汉:华中农业大学, 2019. [25] 成温玉, 赵鸿远, 权群学. 猪δ冠状病毒病原学研究进展[J]. 中国预防兽医学报, 2020,42(9):966-973. DOI:10.3969/j.issn.1008-0589.201912047 [26] Xiu L, Binder RA, Alarja NA, et al.A RT-PCR assay for the detection of coronaviruses from four genera[J]. J Clin Virol, 2020,128:104391. DOI:10.1016/j.jcv.2020.104391 [27] Zhu Y, Liang L, Luo Y, et al.A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens[J]. Virus Genes, 2017,53(1):71-76. DOI:10.1007/s11262-016-1405-z [28] Huang X, Chen J, Yao G, et al.A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses[J]. Appl Microbiol Biotechnol, 2019,103(12):4943-4952. DOI:10.1007/s00253-019-09835-7 [29] 张利卫, 曹贝贝, 李炳晓, 等. 新发猪Delta冠状病毒和猪流行性腹泻病毒SYBR GreenⅠ双重荧光RT-PCR检测方法的建立及应用[J]. 中国兽医学报, 2018,38(4):618-624. [30] Zhu Y, Liang L, Luo Y, et al.A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens[J]. Virus Genes, 2017,53(1):71-76. DOI:10.1007/s11262-016-1405-z [31] 杨靖, 胡姣姣, 柏先泽, 等. 环介导等温扩增技术在病原微生物快速检测中的研究进展[J]. 食品安全质量检测学报, 2018,9(23):6082-6089. DOI:10.3969/j.issn.2095-0381.2018.23.002 [32] Wu X, Song Z, Zhai X, et al.Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick[J]. Poult Sci, 2019,98(11):5401-5411. DOI:10.3382/ps/pez372 [33] Mai TN, Nguyen VD, Yamazaki W, et al.Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay[J]. BMC Vet Res, 2018,14(1):172. DOI:10.1186/s12917-018-1498-9 [34] Zhang F, Ye Y, Song D, et al.A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification[J]. Biol Res, 2017,50(1):30. DOI:10.1186/s40659-017-0135-6 [35] 肖帅, 刘新生, 方玉珍, 等. 猪德尔塔冠状病毒重组聚合酶扩增检测方法的建立与应用[J]. 动物医学进展, 2019,40(3):9-14. DOI:10.3969/j.issn.1007-5038.2019.03.002 [36] 陈坚, 张丹琳, 马磊, 等. 基于重组酶聚合酶扩增技术的犬冠状病毒快速检测方法的建立[J]. 中国兽医杂志, 2020,56(3):88-90. [37] 胡靖飞, 滑翔, 黄小波, 等. 联合共检四种猪腹泻病毒的寡核苷酸芯片的构建及应用[J]. 农业生物技术学报, 2020,28(2):349-359. [38] 李健, 熊炜, 陈沁, 等. 犬猫3种冠状病毒基因克隆的构建与芯片检测技术[J]. 中国兽医学报, 2008,28(11):1284-1287. [39] Oude MB, Sikkema RS, Nieuwenhuijse DF, et al.Transmission of SARS-CoV-2 on mink farms between humans and mink and back to humans[J]. Science, 2021,371(6525):172-177. DOI:10.1126/science.abe5901 [40] Hammer AS, Quaade ML, Rasmussen TB, et al.SARS-CoV-2 transmission between mink (neovison vison) and humans, Denmark[J]. Emerg Infect Dis, 2021,27(2):547-551. DOI:10.3201/eid2702.203794 [41] 翟新验, 刘祥, 原霖, 等. 纳米PCR技术及其在动物疫病检测领域的应用[J]. 中国兽医学报, 2019,39(3):603-608. [42] 林佳琪, 苏国成, 苏文金, 等. 数字PCR技术及应用研究进展[J]. 生物工程学报, 2017,33(2):170-177. DOI:10.13345/j.cjb.160269 [43] Suo T, Liu X, Feng J, et al.ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens[J]. Emerg Microbes Infect, 2020,9(1):1259-1268. DOI:10.1080/22221751.2020.1772678 [44] 侯林杉, 贾敬亮, 顾文源, 等. 基于猪丁型冠状病毒重组S1蛋白间接ELISA抗体检测方法的建立与应用[J]. 畜牧兽医学报, 2019,50(8):1642-1648. DOI:10.11843/j.issn.0366-6964.2019.08.013 [45] 胡林杰, 孟野, 周玉龙, 等. 检测牛冠状病毒抗体间接ELISA方法的建立与应用[J]. 微生物学通报, 2020,47(1):330-338. DOI:10.13344/j.microbiol.china.190422 [46] Hasoksuz M, Hoet AE, Loerch SC, et al.Detection of respiratory and enteric shedding of bovine coronaviruses in cattle in an Ohio feedlot[J]. J Vet Diagn Invest, 2002,14(4):308-313. DOI:10.1177/104063870201400406
2022, Vol. 38 
