Serological surveys and analysis of Marmot Himalayan infected with brucellosis in the Qinghai-Tibet Plateau
YANG Xu-xin1, XU Li-qing1, LI Ji-quan1, YU Shou-hong2, XIE Hui2, XUE Hong-mei1, ZHANG Ai-ping2, REN Ling-ling1, FAN Di3, MA Li1
1. Brucellosis Division,Qinghai Provincial Institute for Endemic Disease Control and Prevention, Xining 810021,China; 2. Department of Plague,Qinghai Provincial Institute for Endemic Disease Control and Prevention, Xining 810021,China; 3. Department of Public Health,Medical College of Qinghai University,Xining 811602,China
Abstract：To determine the geographical distribution map of Qinghai Marmot Himalayan infected with brucellosis based on the detection of the antibody against brucellosis through tiger red agglutination test (RBPT), colloidal gold immunochromatography (GICA) and serum agglutination test ( SAT ). With cross-sectional study of descriptive epidemiology, we collected blood samples of Marmot Himalayan of Qinghai-Tibet Plateau in the areas where brucellosis is prevalent in humans and animals in Qinghai Province, and some counties (cities) where Marmots Himalayan inhabit, used colloidal gold immunochromatography (GICA) , tiger red agglutination test (RBPT) and serum agglutination test ( SAT ) to detect brucellosis. 64 of 1 466 Marmot blood samples were positive for RBPT, with a positive rate of 4.37% (64/1 466). 28 samples were GICA positive, with a positive rate of 1.91% (28/1 466) and SAT positive rate of 1.23%(18/1 466). After pairwise comparison of the detection results of the three methods, the Kappa value of the consistency test results of RBPT and SAT was 0.430, and the comparison of the two methods was moderate consistency. The Kappa value of the consistency test results of GICA and SAT was 0.709, and the strength consistency of the two methods was good. We drawn the geographical distribution map of RBPT, GICA and SAT antibody positive areas based on the tests results, which provided the basis for determining the geographical distribution map of Qinghai Marmot Himalayan infected with brucellosis, and provided references for formulating diversified prevention and control technologies and controlling brucellosis epidemic situation in Qinghai Province.
2022, Vol. 38 
