Abstract：This study investigated Rickettsia infections and genetic characteristics in wild rats in southern and northeastern Hebei Province. Rodents were captured with sticky boards and cages in Handan and Chengde, Hebei. DNA was extracted from the mice spleens. The 16S rRNA, ompA and gltA genes of Rickettsia were amplified with semi-nested PCR. The PCR products were sequenced, the nucleotide identities were determined, and phylogenetic analysis was performed. A total of 100 rodents were captured in Handan City in southern Hebei Province and Chengde City in northern Hebei Province. Six rodents tested positive for the 16S rRNA gene in the spleen, with a positivity rate of 6%. Of them, two were detected in Handan City, and four were detected in Chengde City. In addition, the ompA gene and gltA gene sequences were obtained. Nucleotide identity analysis indicated that all six strains were closely related, and shared the highest nucleotide identity (more than 99.9% for 16S rRNA or gltA, and 99.9% for ompA) with R. raoultii. Phylogenetic analysis using MEGA 6.0 indicated that all six strains had the closest relationship with known R. raoultii strains from other parts of China. Rickettsia infections exist in wild rodents in southern and northeastern Hebei Province. Monitoring and prevention measures for Rickettsia disease should be strengthened to decrease the threats to animal and human health.
刁丹红, 王蒋丽, 郭文平, 李彩云, 杨欢欢, 李雁飞, 谢广成. 河北省野鼠立克次体感染调查[J]. 中国人兽共患病学报, 2022, 38(1): 84-88.
DIAO Dan-hong, WANG Jiang-li, GUO Wen-ping, LI Cai-yun, YANG Huan-huan, LI Yan-fei, XIE Guang-cheng. Investigation of Rickettsia infections in wild rodents in Hebei Province. Chinese Journal of Zoonoses, 2022, 38(1): 84-88.
2022, Vol. 38 
