1. Department of Pathogen and Immunology, Xuzhou Medical University, Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou Key Laboratory of Infection and Immunity, Xuzhou 221004, China; 2. Department of Parasitology of Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
Abstract：This study aimed to investigate the effects and underlying mechanism of Schistosoma japonicum infection on lipid deposition in hepatocytes. Ten mice were randomly divided into a normal group and infected group (n=5). The mice were infected with 15±1 cercariae through the skin. At 6 weeks after infection, liver pathological changes were observed according to HE staining; lipid deposition in liver was observed according to oil red O staining; liver function and lipid levels were detected; and expression of genes associated with lipid metabolism in the liver was detected with real-time quantitative PCR. The liver cell line LO2 was stimulated by the soluble egg antigen SEA. In these cells, lipid deposition was observed through Nile Red staining, and the expression levels of lipid synthesis associated genes were detected by real-time quantitative PCR. In infected mice, the liver showed severe inflammatory granuloma and fibrosis. ALT and AST in the serum increased significantly after infection (tALT=6.432,P<0.01;tAST=3.259,P<0.05). Interestingly, lipid droplets in the liver significantly decreased after infection, and the levels of TG and CHOL also significantly decreased (tTG=7.154,P<0.01;tCHOL=8.749,P<0.001). Compared with those in the normal group, the expression levels of the lipid synthesis related genes Acly, Accs and Fasn were significantly downregulated after infection (tAcly=3.765,P<0.05; tAccs=2.859,P<0.05; tFasn=3.888,P<0.05), whereas the expression levels of the lipid transport related gene CD36 and the lipid catabolism related gene Cpt 1 were upregulated significantly (tCD36=8.250,P<0.01; tCpt1=1.297,P<0.05, respectively). In vitro, as compared with that in the DMEM group, the lipid content of LO2 cells clearly decreased after SEA stimulation, and the expression levels of the lipid synthesis associated genes SREBP-1c, ACLY, ACC and FASN, and the triglyceride synthesis associated genes DGAT and GPAT, all decreased (P<0.05). S. japonicum infection therefore down-regulates lipid deposition in the liver by inhibiting lipid synthesis and promoting lipid lipolysis in hepatocytes.
张波, 郑葵阳, 吴忠道, 张蓓蓓. 日本血吸虫感染对小鼠肝脏脂代谢影响机制的初步研究[J]. 中国人兽共患病学报, 2022, 38(2): 108-114.
ZHANG Bo, ZHANG Kui-yang, WU Zhong-dao, ZHANG Bei-bei. Preliminary study of the mechanism of the effects of Schistosoma japonicum infection on liver lipid metabolism in mice. Chinese Journal of Zoonoses, 2022, 38(2): 108-114.
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2022, Vol. 38 
