Evaluation of the immune efficacy of inactivated whole-cell Campylobacter jejuni vaccine in chickens
WEI Guo-hao1,2, CUI Yi-fang2, GUO Fang-fang2, CAO Xiao-ya2, WANG Xue2, LIU Yan-wei1, XU Fu-zhou2
1. School of Life Sciences and Food Engineering, University of Engineering, Hebei Handan 056038, China; 2. Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Science and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Abstract:On-farm vaccination of poultry against Campylobacter jejuni is considered a potentially effective strategy to decrease human campylobacteriosis. However, no commercial vaccines are currently available to inhibit or reduce the colonization of C. jejuni in poultry. To develop an inactivated whole-cell vaccine for decreasing C. jejuni colonization in chicken intestines, we used a novel inactivated agent and an H2O2 and CuCl2 mixture to kill C. jejuni cells and prepare an oil adjuvant inactivated whole-cell vaccine. SPF chickens were subcutaneously immunized with the inactivated whole-cell vaccine. The effects of the vaccine on the immunized chickens were evaluated on the basis of the induced anti-Campylobacter specific antibody levels, the C. jejuni colonization levels in chicken ceca after oral challenge, the transcriptional levels of cecal cytokines, and the cecal villus length and crypt depth. The inactivated whole-cell vaccine induced significantly higher levels of specific serum IgG and bile IgA in the vaccinated chickens. However, no significant decrease in the colonization level of C. jejuni in chicken ceca was observed between the vaccinated and unvaccinated groups. C. jejuni colonization in the chickens did not significantly affect the cecal villus length and crypt depth. Most cecal cytokines showed no significant difference in transcriptional levels before challenge and 1 day post inoculation (DPI) but a significant increase at 10 DPI. Comparison of cecal samples of the same age indicated that the transcriptional levels of some cytokines in the adjuvant immunized group and the whole-cell immunized group were significantly higher than those in the control group. These results indicated that the inactivated whole-cell vaccine did not decrease the colonization level of C. jejuni in the chicken cecum despite inducing high levels of specific antibodies. The colonization mechanism of C. jejuni in chicken cecum requires further exploration.
魏国昊, 崔一芳, 郭芳芳, 曹晓亚, 王雪, 刘彦威, 徐福洲. 空肠弯曲菌全菌灭活疫苗免疫鸡效果评价[J]. 中国人兽共患病学报, 2022, 38(2): 115-121.
WEI Guo-hao, CUI Yi-fang, GUO Fang-fang, CAO Xiao-ya, WANG Xue, LIU Yan-wei, XU Fu-zhou. Evaluation of the immune efficacy of inactivated whole-cell Campylobacter jejuni vaccine in chickens. Chinese Journal of Zoonoses, 2022, 38(2): 115-121.
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