Effects of IL-25 in the small intestine in mice stimulated with Trichinella spiralis ES antigen
HE Ling1, NA Pisha·jureti1, LIU Xiang-ran1, BAI Jie2
1. College of Graduate, Xinjiang Medical University, Urumqi 830011,China; 2. Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830011,China
Abstract：This study aimed to observe the effects of IL-25 in the small intestine in mice stimulated with Trichinella spiralis ES antigen. A total of 30 BALB/c mice were randomly divided into three groups: control group, ES antigen stimulation group and IL-25 blocking group. The mice in the control group were injected intraperitoneally with PBS; the mice in the ES antigen stimulation group were injected intraperitoneally with ES antigen once per day for 7 consecutive days; and the mice in the IL-25 blocking group were injected intraperitoneally first with anti-mouse IL-25 monoclonal antibody and 3 days later with ES antigen. Enzyme-linked immunosorbent assays were used to detect the changes in the levels of IL-25, IL-13, IL-4, IL-5 and IL-33 in the mouse small intestine tissue homogenate. The pathological changes of the small intestine were observed with HE staining under a microscope; in addition, Alcian Blue-Nuclear fast red staining was used to observe the expression of small intestinal goblet cells. Compared with those in control group, the levels of IL-25 (t=4.675, P<0.05), IL-13 (t=5.392, P<0.05), IL-4 (t=4.275, P<0.05), IL-5 (t=4.675, P<0.05) and IL-33 (t=4.644, P<0.05) in the small intestinal tissue homogenate of mice in the ES antigen stimulation group were higher. Compared with thoes ES antigen stimulation group, the level of IL-25 (t=2.821, P<0.05), IL-13 (t=2.344, P<0.05), IL-4 (t=2.647, P<0.05),IL-5 (t=3.268, P<0.05) and IL-33 (t=2.780, P<0.05) in the small intestine of mice in IL-25 blocking group were lower. Trichinella spiralis ES antigen stimulates the mucosa of the small intestine of mice to produce immune effects through the IL-25/ILC2 pathway.
何灵, 那皮沙·居热提, 刘祥冉, 白杰. IL-25在旋毛虫ES抗原刺激小鼠小肠中的影响[J]. 中国人兽共患病学报, 2022, 38(3): 247-251.
HE Ling, NA Pisha·jureti, LIU Xiang-ran, BAI Jie. Effects of IL-25 in the small intestine in mice stimulated with Trichinella spiralis ES antigen. Chinese Journal of Zoonoses, 2022, 38(3): 247-251.
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2022, Vol. 38 
