Identification and epidemiological characteristics of 525 strains of nontuberculous mycobacteria isolated from a Hunan clinic
LI Wen-bin1, HU Pei-lei1, CHEN Zhong-nan1, CHEN Zheng-hua1, YI Song-lin1, YU Yan-yan1, GUO Jin-wei1, LIU Bin-bin1, WANG Qiao-zhi1, WAN Kang-lin2, TAN Yun-hong1
1. Hunan Institute for Tuberculosis Control/Hunan Chest Hospital, Changsha 410013,China; 2. National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China
Abstract：Nontuberculous mycobacteria (NTM) isolated in Hunan from 2019 to 2020 were identified, and their distribution characteristics were analyzed to provide basic scientific evidence for the prevention and treatment of NTM diseases. Clinical isolates of mycobacteria from patients with suspected tuberculosis at the Hunan Chest Hospital from 2019 to 2020 were collected. The MPB64 protein immunogold method and p-nitrobenzoic acid growth tests were used to identify Mycobacterium tuberculosis complex and NTM. Subsequently, 16S rRNA and Hsp65 sequencing analyses were performed to identify the NTM strains. A total of 6 515 positive cultures of mycobacteria were collected, and 525 strains were initially identified as NTM, accounting for 8.17%. Among them, 285 isolates were from male patients, accounting for 54.29%, and 240 isolates were from female patients, accounting for 45.71%. The population distribution was dominated by farmers, with a total of 367 strains, accounting for 69.90%. Most patients were middle-aged (at least 40 years of age) or older, with 402 strains in total, accounting for 76.57%. Mycobacterium abscessum was mainly prevalent in Chenzhou, Xiangxi Autonomous Prefecture, Hengyang and Yongzhou; intracellular Mycobacterium was prevalent in Chenzhou, Yueyang and Xiangtan. In conclusion, from 2019 to 2020, we identified 27 species of NTM strains, of which the top four were Mycobacterium abscessus (190, 36.19%), Mycobacterium intracellulare (174, 33.14%), Mycobacterium avium bacillus (64, 12.19%) and Mycobacterium gordonii (47, 8.95%). The other 50 strains (9.52%) comprised 23 types of NTM. The detection rate of NTM among mycobacteria in Hunan from 2019 to 2020 remained high, and primarily comprised Mycobacterium abscesses, Mycobacterium intracellulare, Mycobacterium avium and Mycobacterium gordonii. The infected population mainly comprised men, people middle-aged or older, and farmers.
李文彬, 胡培磊, 陈忠南, 陈振华, 易松林, 余艳艳, 郭靖玮, 刘彬彬, 王巧智, 万康林, 谭云洪. 湖南省525株非结核分枝杆菌临床分离株的菌种鉴定与流行特征[J]. 中国人兽共患病学报, 2022, 38(5): 417-422.
LI Wen-bin, HU Pei-lei, CHEN Zhong-nan, CHEN Zheng-hua, YI Song-lin, YU Yan-yan, GUO Jin-wei, LIU Bin-bin, WANG Qiao-zhi, WAN Kang-lin, TAN Yun-hong. Identification and epidemiological characteristics of 525 strains of nontuberculous mycobacteria isolated from a Hunan clinic. Chinese Journal of Zoonoses, 2022, 38(5): 417-422.
2022, Vol. 38 
