成都市人源沙门菌基因组特征分析

doi:10.3969/j.issn.1002-2694.2022.00.059

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (2993 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的基于全基因组测序技术,探究成都市人源沙门菌的基因组特征, 为监测与预防沙门菌感染提供资料。方法收集35株成都市人源沙门菌(分离自腹泻患者粪便)进行全基因组测序。根据测序数据,进行血清型预测、耐药基因及可移动遗传元件预测、毒力因子注释及分布分析。结果 35株沙门菌分离株经全基因组测序,共预测到5种血清型,包括15株I 4,[5],12:i:-沙门菌(13株为ST34、2株为新ST型)、12株鼠伤寒沙门菌(ST19)、5株肠炎沙门菌(ST11)、2株德比沙门菌(ST40)与1株火鸡沙门菌(ST463)。35株沙门菌分离株共预测到10类42种不同的耐药基因,其中氨基糖苷类aac(6')-Iaa携带率为100%(35/35)。I 4,[5],12:i:-沙门菌的耐药基因、质粒及插入序列数目及种类多于其他血清型菌株。I 4,[5],12:i:-、鼠伤寒沙门菌和肠炎沙门菌的毒力因子数目大于其他血清型菌株。部分鼠伤寒沙门菌有更高的毒力质粒、质粒编码菌毛和血清抗性毒力因子分布。结论成都市人源沙门菌不同血清型菌株之间耐药基因、可移动遗传元件、毒力因子分布差异明显,值得在转录组、蛋白组进一步探究其耐药与毒力机制。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	肖颖
	武雅婷
	赵婉妤
	崔学文
	黎明
	许欣

关键词 ：沙门菌, 全基因组测序, 耐药基因, 毒力特征

Abstract：We investigated the genomic characteristics of Salmonella in humans in Chengdu by using whole genome sequencing to provide a basis for the surveillance and prevention of Salmonella infection. Whole genome sequencing was performed on 35 Salmonella strains from humans in Chengdu, which were isolated from the feces of patients with diarrhea. According to the sequencing data, serotypes, drug resistance genes and mobile genetic elements were identified, virulence factors (VF) were annotated, and their distribution was analyzed. Five serotypes were predicted among the 35 tested Salmonella strain isolates: 15 S.I 4,[5],12:i:-(ST34, two strains with unknown ST type), 12 S. typhimurium (ST19), 5 S. enteritidis (ST11), 2 S. derby (ST40) and 1 S. meleagridis (ST463). Ten drug resistance phenotypes (42 drug resistance genes) were predicted, and the aac(6')-Iaa gene was predicted in all Salmonella strains. The S.I 4,[5],12:i:-had more drug resistance genes, plasmids and insert sequences. The S.I 4,[5],12:i:-, S. typhimurium and S. enteritidis had higher numbers of VF than the other strains. Some S. typhimurium had higher levels of Salmonella plasmid virulence, plasmid-encoded fimbriae and serum resistance. The drug resistance genes, mobile genetic elements and distribution of VF significantly differed among the five Salmonella serotype strains in humans in Chengdu. The resistance and virulence mechanisms of Salmonella require further exploration at the transcriptomic and proteomic levels.

Key words： Salmonella whole genome sequencing drug resistance genes virulence characteristics

收稿日期: 2021-11-08

R378.2+2

基金资助:国家重点研发计划项目(No.2018YFC1603900)

通讯作者: 许欣,Email:xinxu62@163.com; ORCID:0000-0001-7280-9501

引用本文:

肖颖, 武雅婷, 赵婉妤, 崔学文, 黎明, 许欣. 成都市人源沙门菌基因组特征分析[J]. 中国人兽共患病学报, 2022, 38(5): 433-440. XIAO Ying, WU Ya-ting, ZHAO Wan-yu, CUI Xue-wen, LI Ming, XU Xin. Analysis of genomic characteristics of Salmonella in humans in Chengdu. Chinese Journal of Zoonoses, 2022, 38(5): 433-440.

链接本文:

http://www.rsghb.cn/CN/10.3969/j.issn.1002-2694.2022.00.059 或 http://www.rsghb.cn/CN/Y2022/V38/I5/433

[1] 杨小蓉, 周良君, 陈文, 等. 2010-2018年四川省沙门菌食源性疾病暴发分析[J]. 现代预防医学, 2020, 47(5): 769-771,775.
[2] Issenhuth-Jeanjean S, Roggentin P, Mikoleit M, et al.Supplement 2008-2010 (no. 48) to the White-Kauffmann-LeMinor scheme[J]. Res Microbiol, 2014, 165(7): 526-530. DOI: 10.1016/j.resmic.2014.07.004
[3] Gymoese P, Sorensen G, Litrup E, et al.Investigation of outbreaks of Salmonella enterica serovar typhimurium and its monophasic variants using whole-genome sequencing, Denmark[J]. Emerg Infect Dis, 2017, 23(10): 1631-1639. DOI: 10.3201/eid2310.161248
[4] Seif Y, Kavvas E, Lachance J C, et al.Genome-scale metabolic reconstructions of multiple Salmonella strains reveal serovar-specific metabolic traits[J]. Nat Commun, 2018, 9(1): 3771. DOI: 10.1038/s41467-018-06112-5
[5] Tay M Y F, Pathirage S, Chandrasekaran L, et al. Whole-genome sequencing analysis of nontyphoidal Salmonella enterica of chicken meat and human origin under surveillance in Sri Lanka[J]. Foodborne Pathog Dis, 2019, 16(7): 531-537. DOI: 10.1089/fpd.2018.2604
[6] 张璐, 沈青春, 张纯萍, 等. 全基因组测序技术对沙门氏菌血清型和耐药性的预测能力分析[J]. 微生物学报,2021, 61(12): 4038-4047. DOI: 10.13343/j.cnki.wsxb.20210140
[7] 杨小鹃, 吴清平, 张菊梅, 等. 沙门氏菌1,4,[5],12:i:-耐药性和遗传特征研究进展[J]. 微生物学报, 2014, 54(11): 1248-1255. DOI: 10. 13343/j. cnki. wsxb. 2014. 11. 002
[8] 柯碧霞, 曾洪辉, 何冬梅, 等. 广东省2007-2016年人源沙门菌流行现状及病原学特征[J]. 中华流行病学杂志, 2018, 39(1): 63-66. DOI: 10.3760/cma.j.issn.0254-6450.2018.01.013
[9] 黄水飞, 彭淑萍, 许铭清, 等. 2017-2019年茂名市I4,[5],12∶i∶-沙门菌流行特征及耐药性分析[J]. 中国食品卫生杂志, 2020, 32(6): 681-685. DOI: 10. 13590/j.cjfh.2020. 06. 017
[10] 仇绮琳, 郑悦康, 刘绮明, 等. 广东省中山市2018-2019年沙门菌血清型分布、耐药性及分子分型[J]. 中国热带医学, 2021, 21(8): 758-763. DOI: 10.13604/j.cnki.46-1064/r.2021.08.09
[11] Hong S, Rovira A, Davies P, et al.Serotypes and antimicrobial resistance in Salmonella enterica recovered from clinical samples from cattle and swine in Minnesota, 2006 to 2015[J]. PLoS One, 2016, 11(12): e0168016. DOI: 10.1371/journal.pone.0168016
[12] Yuan C, Krull A, Wang C, et al.Changes in the prevalence of Salmonella serovars associated swine production and correlations of avian, bovine and swine-associated serovars with human-associated serovars in the United States (1997-2015)[J]. Zoonoses Public Health, 2018, 65(6): 648-661. DOI: 10.1111/zph.12473
[13] 黄静敏, 柯碧霞, 李柏生, 等. 广东省多重耐药沙门菌1,4,[5],12∶i∶-的耐药性及分子流行特征分析[J]. 疾病监测, 2021, 36(5): 501-508. DOI:10.3784/jbjc.202012020405
[14] Oliva M, Monno R, D’addabbo P, et al.A novel group of IncQ1 plasmids conferring multidrug resistance[J]. Plasmid, 2017, 89: 22-26. DOI: 10.1016/j.plasmid.2016.11.005
[15] 方艳红, 孙裴, 魏建忠, 等. 沙门菌毒力基因研究进展[J]. 动物医学进展, 2010, 31(S1): 190-193. DOI: 10.16437/j.cnki.1007-5038.2010.s1.073
[16] Krause M, Fang FC, Guiney DG.Regulation of plasmid virulence gene expression in Salmonella dublin involves an unusual operon structure[J]. J Bacteriol, 1992, 174(13): 4482-4489. DOI: 10.1128/jb.174.13.4482-4489.1992
[17] Paesold G, Guiney DG, Eckmann L, et al.Genes in the Salmonella pathogenicity island 2 and the Salmonella virulence plasmid are essential for Salmonella-induced apoptosis in intestinal epithelial cells[J]. Cell Microbiol, 2002, 4(11): 771-781. DOI: 10.1046/j.1462-5822.2002.00233.x
[18] Li H, Xu H, Zhou Y, et al.The phosphothreonine lyase activity of a bacterial type III effector family[J]. Science, 2007, 315(5814): 1000-1003. DOI: 10.1126/science.1138960
[19] Guiney DG, Fierer J.The role of the spv genes in Salmonella pathogenesis[J]. Front Microbiol, 2011, 2: 129. DOI: 10.3389/fmicb.2011.00129
[20] Passaris I, Cambre A, Govers SK, et al.Bimodal expression of the Salmonella typhimurium spv operon[J]. Genetics, 2018, 210(2): 621-635. DOI: 10.1534/genetics.118.300822
[21] Kuijpers AFA, Bonacic Marinovic AA, Wijnands LM, et al.Phenotypic prediction: linking in vitro virulence to the genomics of 59 Salmonella enterica strains[J]. Front Microbiol, 2018, 9: 3182. DOI: 10.3389/fmicb.2018.03182
[22] Zhao S, Li C, Hsu CH, et al.Comparative genomic analysis of 450 strains of Salmonella enterica isolated from diseased animals[J]. Genes (Basel), 2020, 11(9):1025. DOI: 10.3390/genes11091025
[23] Nicholson B, Low D.DNA methylation-dependent regulation of pef expression in Salmonella typhimurium[J]. Mol Microbiol, 2000, 35(4): 728-742. DOI: 10.1046/j.1365-2958.2000.01743.x
[24] Rosselin M, Virlogeux-Payant I, Roy C, et al.Rck of Salmonella enterica, subspecies enterica serovar enteritidis, mediates zipper-like internalization[J]. Cell Res, 2010, 20(6): 647-664. DOI: 10.1038/cr.2010.45
[25] Craig M, Slauch JM.Phagocytic superoxide specifically damages an extracytoplasmic target to inhibit or kill Salmonella[J]. PLoS One, 2009, 4(3): e4975. DOI: 10.1371/journal.pone.0004975
[26] Krishnakumar R, Craig M, Imlay JA, et al.Differences in enzymatic properties allow SodCI but not SodCII to contribute to virulence in Salmonella enterica serovar typhimurium strain 14028[J]. J Bacteriol, 2004, 186(16): 5230-5238. DOI: 10.1128/JB.186.16.5230-5238.2004