Diversity analysis of endosymbiotic fungi in ticks of Haemaphysalis spp. in the tropical environment by Illumina MiSeq
LU Ya-jun1,2, YUAN Chuan-fei1, XIE Yun-yun1, XIA Qi1, XIA Qian-feng1
1. School of Tropical Medicine, Hainan Medical University, Haikou 571199, China; 2. School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710049, China;
Abstract：This study investigated the community composition and analyzed the diversity of the endosymbiotic fungi in adult Haemaphysalis spp. ticks in tropical environments in Hainan. Adult tick samples were morphologically classified, and the mitochondrial COI gene was amplified for molecular identification. The endosymbiotic fungal ITS1 genes in Haemaphysalis spp. samples were sequenced on the Illumina MiSeq platform, and the community composition and diversity of the endosymbiotic fungi were assessed with OTU cluster analysis and alpha diversity analysis. The endosymbiotic fungi in Haemaphysalis spp. in tropical environments clustered into 245 OTUs. The community composition was distributed among Ascomycota (80.56%), Basidiomycota (15.43%), and Zygomycota (0.37%); the dominant species was Zasmidium citrigriseum, which accounted for 7.72%. Alpha diversity analysis indicated that the endosymbiotic fungi in Haemaphysalis spp. had a rich biodiversity. This study provides a basis for revealing the complex interactions between ticks and endosymbiotic fungi, and provides new ideas for further elucidating the behavioral characteristics of tropical ticks and exploring effective biological control strategies.
芦亚君, 袁传飞, 解芸芸, 夏琪, 夏乾峰. Illumina MiSeq对热带环境血蜱属成蜱内共生真菌多样性分析[J]. 中国人兽共患病学报, 2022, 38(5): 441-446.
LU Ya-jun, YUAN Chuan-fei, XIE Yun-yun, XIA Qi, XIA Qian-feng. Diversity analysis of endosymbiotic fungi in ticks of Haemaphysalis spp. in the tropical environment by Illumina MiSeq. Chinese Journal of Zoonoses, 2022, 38(5): 441-446.
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