PFGE analysis of leptospirosis in different serum groups
LI Zhe, ZHANG Ying, DU Zong-li, YE Qiang, XU Ying-hua, XIN Xiao-fang
Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products,National Institute of Food and Drug Control,Beijing 102629,China
Abstract:To understand the zone characteristics of pulsed-field gel electrophoresis (PFGE) of pathogenic leptospires in different serum groups, the PFGE method was used to classify 65 international and domestic reference strains and vaccine strains of pathogenic leptospires with different serogroups. Cluster analysis was carried out with multilocus sequence typing (MLST) and multilocus VNTR analysis (MLVA). Using Not I enzyme digestion electrophoresis, a high definition enzyme digestion map of pathogenic Leptospira genomic DNA of different serum groups was obtained; 65 strains of Leptospira were divided into 61 PFGE types. The PFGE types of Leptospira strains in the same serum group were different. The results of comparative analysis showed that, although the typing results of PFGE, MLST and MLVA were different, they all indicated a high rate of differentiation. Cluster analysis demonstrated that these strains could form 11 evolutionary clusters of different sizes, showing the characteristics of multi-point dispersion evolution. The primary data of molecular typing of pathogenic leptospires can provide scientific guidance for molecular tracing and epidemiological study.
李喆, 张影, 杜宗利, 叶强, 徐颖华, 辛晓芳. 不同血清群致病性钩端螺旋体PFGE分析研究[J]. 中国人兽共患病学报, 2022, 38(6): 481-485.
LI Zhe, ZHANG Ying, DU Zong-li, YE Qiang, XU Ying-hua, XIN Xiao-fang. PFGE analysis of leptospirosis in different serum groups. Chinese Journal of Zoonoses, 2022, 38(6): 481-485.
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