Abstract：To detect the pathogen involved in a cluster of cases of severe fever with thrombocytopenia syndrome (SFTS) from Nanjing Gulou Hospital in April 2020, the S gene of the pathogen was sequenced, and the genotype and phylogenetic characteristics were studied. Nucleic acid qualitative detection of SFTSV was carried out by the fluorescence quantitative PCR method in six cases with serum samples taken in the acute stage. The S fragments of positive samples were amplified by RT-PCR and sequenced. Nucleotide homology was analyzed and a phylogenetic tree was constructed. Five of the six serum samples were positive for SFTSV, and the five cases were highly homologous: four had 100% homology of the S fragment nucleotide sequence, and one case had 99.7% homology with the other four cases. The most similar strains were isolated from Jiangsu in 2014 (JS2014-06) and 2015(JS2015-26), with 100% homology. The five cases were all in a branch of the C2 subtype. The SFTSV genotype of this cluster outbreak is C2 and its nucleotides are highly homologous with domestic isolates in recent years. Monitoring and education need to be strengthened and we should pay attention to the evolution and mutation of the virus.
何敏, 董晓庆, 石利民, 王雅倩, 张守刚. 一起聚集性发热伴血小板减少综合征病原S基因分子进化分析[J]. 中国人兽共患病学报, 2022, 38(6): 502-506.
HE Min, DONG Xiao-qing, SHI Li-min, WANG Ya-qian, ZHANG Shou-gang. Phylogenetic analyses of the S gene of SFTSV in a cluster of cases of severe fever with thrombocytopenia syndrome. Chinese Journal of Zoonoses, 2022, 38(6): 502-506.
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2022, Vol. 38 
