Abstract：Molecular characteristics of the whole genomes of dengue serotype 1 virus (DENV-1) strains isolated from dengue fever cases in Shanghai during 2018-2020 were investigated. DENV-1 was isolated by using the Vero-SLAM cell culture method. Full-length genomes of DENV-1 were amplified by reverse transcriptase polymerase chain reaction and sequences were further researched through phylogenetic analysis, nucleotide and amino acid sequence identities and amino acid site analysis. From 88 serum samples, 31 strains of DENV-1 were successfully isolated, 3 were from indigenous cases, 28 were from imported cases. Phylogenetic analysis revealed that 28 isolates belonged to genotype I (G-I). Compared with reference strains, nucleotide and amino acid sequence identities were 96.47%-97.37% and 98.78%-99.16%, respectively. Three strains belonged to genotype IV (G-IV), the nucleotide and amino acid sequence identities of which were 96.66%-96.86% and 99.01%-99.26%, respectively. The genotype of strains isolated from indigenous cases was G-I, phylogenetic analysis of which suggested possible transmission from imported cases. Compared with the reference strain, there were different sites among amino acid residues of all structural and non-structural proteins. Of 495 amino acid residues of the E protein, there were 31 sites with differences.
王葳, 牟嘉斌, 王环茹, 房方皓, 朱奕奕, 滕峥. 2018-2020年上海市登革1型病毒全基因组序列特征研究[J]. 中国人兽共患病学报, 2022, 38(6): 507-514.
WANG Wei, MOU Jia-bin, WANG Huan-ru, FANG Fang-hao, ZHU Yi-yi, TENG Zheng. Molecular characteristics of the whole genomes of DENV-1 strains isolated from dengue fever cases in Shanghai, China, from 2018 to 2020. Chinese Journal of Zoonoses, 2022, 38(6): 507-514.
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