Research progress on the adhesion mechanism and genotyping of intimin in enteropathogenic Escherichia coli
YANG Lei1,2, LI Jun-lin1,2, SHEN Yu-xi1,2, LUO Yu-wen1,2, XIA Jing1,2, HUANG Yong1,2, YAN Qi-gui1,2, ZOU Li-kou3, HAN Xin-feng1,2
1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2. Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, China; 3. College of Resources, Sichuan Agricultural University, Chengdu 611130, China
Abstract：Diarrheal diseases caused by enteropathogenic Escherichia coli (EPEC) pose a challenge to public health worldwide. During the adhesion of EPEC to intestinal cells, the interaction of intimin with the translocation receptor Tir triggers the assembly of actin, forming a tightly adherent base at the site of bacterial-host cell attachment. Intimin is not only an essential key factor allowing EPEC to produce characteristic attaching-and-effacing lesions, but also has different tissue orientation to the host. The intimin encoding gene eae, a target for identifying EPEC strains, has considerable heterogeneity and is an important genetic basis for EPEC typing. Moreover, LEE pathogenicity islands carrying different eae subtypes may be transferred horizontally among different serotypes of diarrheagenic E. coli strains. Thus, identification and typing of the eae gene of EPEC strains could provide a reference for surveillance and prediction of severity in EPEC diarrheal epidemics.
杨蕾, 李俊霖, 申玉玺, 罗钰雯, 夏静, 黄勇, 颜其贵, 邹立扣, 韩新锋. 肠致病性大肠杆菌紧密黏附素的作用机制及基因分型研究进展[J]. 中国人兽共患病学报, 2022, 38(6): 528-532.
YANG Lei, LI Jun-lin, SHEN Yu-xi, LUO Yu-wen, XIA Jing, HUANG Yong, YAN Qi-gui, ZOU Li-kou, HAN Xin-feng. Research progress on the adhesion mechanism and genotyping of intimin in enteropathogenic Escherichia coli. Chinese Journal of Zoonoses, 2022, 38(6): 528-532.
[1] Oh J Y, Kang M S, An B K, et al.Prevalence and characteristics of intimin-producing Escherichia coli strains isolated from healthy chickens in Korea[J]. Poult Sci, 2012, 91(10): 2438-2443. DOI: 10.3382/ps.2012-02301 [2] Yang S C, Lin C H, Aljuffali I A, et al.Current pathogenic Escherichia coli foodborne outbreak cases and therapy development[J]. Arch Microbiol, 2017, 199(6): 811-825. DOI: 10.1007/s00203-017-1393-y [3] Hua Y, Bai X, Zhang J, et al.Molecular characteristics of eae-positive clinical Shiga toxin-producing Escherichia coli in Sweden[J]. Emerg Microbes Infect, 2020, 9(1): 2562-2570. DOI: 10.1080/22221751.2020.1850182 [4] Gomes TA, Elias WP, Scaletsky IC, et al.Diarrheagenic Escherichia coli[J]. Braz J Microbiol, 2016, 47(Suppl 1):3-30. DOI: 10.1016/j.bjm.2016.10.015 [5] Schmidt MA.LEEways: tales of EPEC, ATEC and EHEC[J]. Cell Microbiol, 2010, 12(11): 1544-1552. DOI: 10.1111/j.1462-5822.2010.01518.x [6] Hu J, Torres AG.Enteropathogenic Escherichia coli: foe or innocent bystander[J]. Clin Microbiol Infect, 2015, 21(8): 729-734. DOI: 10.1016/j.cmi.2015.01.015 [7] Pearson J S, Giogha C, Wong Fok Lung T, et al. The genetics of enteropathogenic Escherichia coli virulence[J]. Annu Rev Genet, 2016, 50: 493-513. DOI: 10.1146/annurev-genet-120215-035138 [8] Bhatt S.Atypical enteropathogenic Escherichia coli: from kittens to humans and beyond[J]. Infect Immun, 2021, 89(3). DOI: 10.1128/IAI.00752-20 [9] Fairman J W, Dautin N, Wojtowicz D, et al.Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis[J]. Structure, 2012, 20(7): 1233-1243. DOI: 10.1016/j.str.2012.04.011 [10] Ferdous M, Kooistra-Smid AM, Zhou K, et al.Virulence, antimicrobial resistance properties and phylogenetic background of non-H7 enteropathogenic Escherichia coli O157[J]. Front Microbiol, 2016, 7: 1540. DOI: 10.3389/fmicb.2016.01540 [11] Fitzhenry RJ, Pickard DJ, Hartland EL, et al.Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7[J]. Gut, 2002, 50(2): 180-185. DOI: 10.1136/gut.50.2.180 [12] Tzipori S, Gunzer F, Donnenberg M S, et al.The role of the eaeA gene in diarrhea and neurological complications in a gnotobiotic piglet model of enterohemorrhagic Escherichia coli infection[J]. Infect Immun, 1995, 63(9): 3621-3627. DOI: 10.1128/iai.63.9.3621-3627.1995 [13] Heinz E, Stubenrauch C J, Grinter R, et al.Conserved features in the structure, mechanism, and biogenesis of the inverse autotransporter protein family[J]. Genome Biol Evol, 2016, 8(6): 1690-1705. DOI: 10.1093/gbe/evw112 [14] Xu Y, Bai X, Zhao A, et al.Genetic Diversity of intimin gene of atypical enteropathogenic Escherichia coli isolated from human, animals and raw meats in China[J]. PLoS One, 2016, 11(3): e0152571. DOI: 10.1371/journal.pone.0152571 [15] Katani R, Cote R, Raygoza Garay J A, et al. Complete genome sequence of SS52, a strain of Escherichia coli O157:H7 recovered from supershedder cattle[J]. Genome Announc, 2015, 3(2):e01569-14. DOI: 10.1128/genomeA.01569-14 [16] Huang Y, Shan X F, Deng H, et al.Epidemiology, antimicrobial resistance and beta-lactamase genotypic features of enteropathogenic Escherichia coli isolated from children with diarrhea in Southern China[J]. Jpn J Infect Dis, 2015, 68(3): 239-243. DOI: 10.7883/yoken.JJID.2014.120 [17] Yang K, Pagaling E, Yan T.Estimating the prevalence of potential enteropathogenic Escherichia coli and intimin gene diversity in a human community by monitoring sanitary sewage[J]. Appl Environ Microbiol, 2014, 80(1): 119-127. DOI: 10.1128/AEM.02747-13 [18] Vieira MA, Dos Santos LF, Dias RCB, et al.Atypical enteropathogenic Escherichia coli as aetiologic agents of sporadic and outbreak-associated diarrhoea in Brazil[J]. J Med Microbiol, 2016, 65(9): 998-1006. DOI: 10.1099/jmm.0.000313 [19] Lacher DW, Steinsland H, Whittam TS.Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP[J]. FEMS Microbiol Lett, 2006, 261(1): 80-87. DOI: 10.1111/j.1574-6968.2006.00328.x [20] Zhang S, Yang G, Huang Y, et al.Prevalence and characterization of atypical enteropathogenic Escherichia coli isolated from retail foods in China[J]. J Food Prot, 2018, 81(11): 1761-1767. DOI: 10.4315/0362-028X.JFP-18-188 [21] Yang X, Sun H, Fan R, et al.Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China[J]. Sci Rep, 2020, 10(1): 3275. DOI: 10.1038/s41598-020-60225-w [22] De Almeida PM, Arais LR, Andrade JR, et al.Characterization of atypical enteropathogenic Escherichia coli (aEPEC) isolated from dogs[J]. Vet Microbiol, 2012, 158(3/4): 420-424. DOI: 10.1016/j.vetmic.2012.02.021 [23] Ramachandran V, Brett K, Hornitzky MA, et al.Distribution of intimin subtypes among Escherichia coli isolates from ruminant and human sources[J]. J Clin Microbiol, 2003, 41(11): 5022-5032. DOI: 10.1128/JCM.41.11.5022-5032.2003 [24] Tarr CL, Whittam TS.Molecular evolution of the intimin gene in O111 clones of pathogenic Escherichia coli[J]. J Bacteriol, 2002, 184(2): 479-487. DOI: 10.1128/JB.184.2.479-487.2002
2022, Vol. 38 
