Genomic analysis of a strain of Streptococcus suis serotype 9
XI Xiao-yan1, LI Xiao-qi2, CHEN Jia-yuan2, PENG Ling2
1. Medical College, Shaoguan University, Shaoguan 512026, China; 2. Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China
Abstract:In this study, the whole genome sequence of the virulent strain of Streptococcus suis serotype 9 GD18 was annotated through bioinformatics methods, and comparative genomics analysis was performed with 15 strains of S. suis in GenBank. The genome of GD18 is 2 067 661 bp, with 1 961 proteins. The genes annotated in COG, GO, KEGG, CAZy and PHL-Base accounted for 77.05%, 71.29%, 43.91%, 4.49% and 3.37% of GD18 total protein, respectively. Virulence factor analysis indicated that the virulence factors of S. suis were complex, and not all S. suis strains shared a set of common virulence factors. Drug resistance gene analysis revealed that GD18 contains many drug resistance genes and has a rich drug efflux system. GD18 contains 114 signal peptide proteins and 30 secretory proteins. A total of 1 244 core genes, 1 685 dispensable genes and 1 417 specific genes were identified in 16 S. suis strains through comparative genome analysis. Phylogenetic analysis indicated that GD18 was most closely related to the Canadian strain 89-3576-3, and the domestic strains were located in different branches. Therefore, the genetic relationships among S. suis strains are complex, and their evolutionary pathway is diverse in China. This study lays a foundation and provides data support for research on the overall level of the genome of S. suis serotype 9.
席小燕, 李晓琪, 陈家苑, 彭凌. 1株9型猪链球菌全基因组分析[J]. 中国人兽共患病学报, 2022, 38(8): 707-713.
XI Xiao-yan, LI Xiao-qi, CHEN Jia-yuan, PENG Ling. Genomic analysis of a strain of Streptococcus suis serotype 9. Chinese Journal of Zoonoses, 2022, 38(8): 707-713.
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