生猪养殖环境及猪肉中金黄色葡萄菌肠毒素基因分布研究

doi:10.3969/j.issn.1002-2694.2018.00.067

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Abstract The aim of this study is to investigate the prevalence of staphylococcal enterotoxin genes profile of Staphylococcus aureus in pig farms samples and commercial fresh pork. Totally 18 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser, seu) were detected in S. aureus by PCR. Of 130 S. aureus, 94.6%(123/130)isolates were positive for enterotoxin genes, including 55%(55/100)isolates from pig farms samples and 80%(24/30)isolates from commercial fresh pork. The highest positive rate of seb account for 60.8% among 18 enterotoxin genes. The main toxins gene profiles were detected in S.aureus from pig farms samples were seb+seg+sem+sen, seg+sem, seg+sei+sem+sen, seg+sem+sen and seb+seg+sei+sem+sen, and their positive rates were 8.0%(8/100), 7.0%(7/100), 7.0%(7/100)and 6.0%(6/100)respectively. The main toxins gene profiles were detected in S.aureus from commercial fresh pork were sea+seb+see, sea+seb, seb+see and sea+see+sem, and their positive rates were 30%(9/30), 10%(3/30), 10%(3/30)and 6.7%(2/30)respectively. Neither sed, sej, seo, sep, seq, ser or seu genes were detected in 130 S. aureus. S. aureus were isolated from pig farms and commercial fresh pork carrying toxin genes, and the toxins gene profiles were different, there may be different pollution sources. Our findings has certain significance for monitoring the status of S. aureus pollution and the distribution of toxin genes in pig farms and commercial fresh pork.

Key words： pig farm commercial fresh pork Staphylococcus aureus staphylococcal enterotoxin genes

Received: 28 September 2017

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R378.1

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	ZOU Zhong-ai

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ZOU Zhong-ai. Detection of staphylococcal enterotoxin genes in Staphylococcus aureus isolates from pig farms and commercial fresh pork[J]. Chinese Journal of Zoonoses, 2018, 34(5): 441-446.

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http://www.rsghb.cn/EN/10.3969/j.issn.1002-2694.2018.00.067 OR http://www.rsghb.cn/EN/Y2018/V34/I5/441

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