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Molecular characterization and phylogenetic analysis of the envelope gene of dengue virus type 3 in Guangzhou, China, 2001-2016 |
LIU Yuan, JIANG Li-yun, JING Qin-long, SU Wen-zhe, CAI Wen-feng, DI biao, YANG Zhi-cong |
Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China |
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Abstract We investigated the epidemiological characteristics of dengue virus serotype 3 in Guangzhou. By analyzing the E gene of the isolated strains, phylogenetic tree were constructed to know about the evolution and trend of the strains. Strains were isolated from the sera, which were detected positive by real-time PCR, of dengue fever patients using the C6/36 cell line.E gene of the isolated strains were sequenced and compared with the sequences in NCBI. Phylogenetic tree was drawn by Mega 4.0. Twenty-four strains were isolated in 2001-2016. The strains clustered in genotype I,II,III and V. Genotype III was predominate. The strains isolated in Guangzhou showed epidemiology connection and a high identity to the Southeast Asian strains. Dengue virus serotype 3 in Guangzhou is mainly imported. There are close connections between the Guangzhou strains and Southeast Asian strains. Guangzhou is in a high risk of imported dengue fever, and outbreak of dengue haemorrhagic fever and dengue shock syndrome. Increase of genotypes and shifts between genotypes may make the epidemic of DENV3 in Guangzhou more complex and raise the risk of outbreaks.
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Received: 30 June 2017
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Corresponding Authors:
Yang Zhi-cong, Email: yangzc@gzcdc.org.cn
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