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| Preparation and identification of monoclonal antibody against Zaire Ebola virus |
| LIU Rong-jiao1, WANG Ren-xia3, LI Zi-wei1, BIN Lei1, TAN Ya-fang2, WANG Jin2, DU Zong-min2, DENG Zhong-liang1 |
1. School of Public Health, University of South China, Hengyang 421001, China;
2. Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military MedicalSciences, Beijing 100071, China;
3. Clinical Laboratory of Center for Disease Prevention and Control of Zhuzhou,Zhuzhou 412000, China |
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Abstract Zaire Ebola virus nucleoprotein (EBOV NP) synthetic peptide was used as the immunogen to prepare mouse monoclonal antibody, and the specificity of prepared monoclonal antibody was tested with recombinant nucleoproteins of four Ebola virus epidemic strains.The artificially synthesized nucleoprotein peptides of Zaire Ebola virus were used to immunize animals, and hybridoma cell lines were screened after the cells fusion.Four eukaryotic expression vectors of the Ebola virus subtype were constructed to transfect HEK293T cell to express target proteins. Then the eukaryotic expression proteins were used as antigens to test the specificity of prepared monoclonal antibody against Zaire EBOV by Western blotting. We accomplished the preparation of monoclonal antibody against Zaire EBOV, and twelve cell lines that can secret monoclonal antibodies against Zaire EBOV were obtained,the titers of antibodies in mouse ascites were among 1∶104-1∶105. Three hybridoma cell lines of the twelve were screened, which secret antibodies that have no cross reaction with other three Ebola virus subtype, and these antibodies had high titer and specificity. In conclusion, the monoclonal antibody against Zaire Ebola virus was successfully prepared, which laid the foundation for the rapid detection of Ebola virus.
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Received: 18 January 2018
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| Fund:Supported by the Human Provincial Department of Education Key Projects(No. 15A165) |
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Corresponding Authors:
Deng Zhong-liang, Email: dzl021015@sina.com
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2018, Vol. 34 
