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Generation of bacterial ghost from Escherichia coli in ducklings by expression of Lysis gene E and Staphylococcal nuclease A gene |
PENG Ling1,2, YANG Xu-fu1,2 |
1.Yingdong College of Life Science,Shaoguan University,Shaoguan 512005,China; 2.Joint Laboratory of Animal Infectious Diseases Diagnostic Center-Harbin Veterinary research Institute of Chinese Academy of Agriculture Science,Shaoguan University,Shaoguan 512005,China |
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Abstract We generated bacterial ghost from Escherichia coli in ducklings by expression of Lysis gene E and Staphylococcal nuclease A gene. Plasmid pET29a-E-S consisting of E gene and Staphylococcal nuclease A gene was transferred into E.coli O92 from ducklings. E.coli O92(pET29a-E-S) was induced to lyse and the OD600 value of culture media was measured every 30 min during the induction, and DNA in medium were detected. The result showed the OD600 value of E.coli O92(pET29a-E-S) began to decline after 90 min of induction, after 180 min of induction, the OD600 value decline speed became slowly, and after 360 min of induction, the rate of genetic inactivation was at 99.999%. DNA in medium was degraded into small fragments (50 bp-400 bp) by Staphylococcus nuclease A. The E.coli O92 ghost was generated successfully by expression of Lysis E gene and Staphylococcal nuclease A gene, which laid a foundation on the study of bacterial ghost vaccine against E.coli O92 infection.
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Received: 04 September 2017
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Fund:Supported by the Key Supporting Disciplines of Ecology in Shaoguan University(No.230079030101) |
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