肺结核患者外周血免疫细胞水平与临床意义分析

doi:10.3969/j.issn.1002-2694.2018.00.106

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Abstract We investigated the expression level and clinical significance of immune cells in peripheral blood of patients with pulmonary tuberculosis. Data from 676 patients with pulmonary tuberculosis and 40 healthy controls collected from January 2016 to May 2017 was analyzed retrospectively. The absolute number of CD4⁺, CD8⁺, B and NK cells in peripheral blood were detected with FC500 flow cytometry, fluorescent microspheres used as a reference. Results showed that the number of CD4⁺, CD8⁺, B, NK cells in patients with pulmonary tuberculosis was significantly lower than that of healthy controls (t= 6.25, 5.12, 5.05, 4.37; P=0.00 each). The number of CD4⁺, CD8⁺, B, NK cells in sputum-positive patients with pulmonary tuberculosis was significantly lower than that in sputum-negative patients (t=4.07,3.70,2.75,2.71;P=0.00,0.00,0.01,0.01).The number of CD4⁺, CD8⁺ and B cells in elderly patients with pulmonary tuberculosis was significantly lower than in young patients(t=4.04,2.60,5.25;P=0.00,0.01,0.00). After 2 months chemotherapy about 554 initial treatment pulmonary tuberculosis, the number of CD4⁺, CD8⁺, B and NK cells did not increase significantly in 510 pulmonary tuberculosis patients with standardized anti-tuberculosis simply (t=1.19,1.04,0.39,1.82;P=0.23,0.30,0.70,0.07); the number of CD4⁺,CD8⁺ T lymphocytes and B lymphocytes increased significantly in 44 pulmonary tuberculosis patients after 2 months chemotherapy combined with immunotherapy(t=2.15,2.17,3.12;P=0.03,0.03,0.00),while the number of NK cells did not increase significantly (t= 0.80; P=0.42). The expression levels of immune cells decreased in patients with pulmonary tuberculosis, especially in the sputum positive pulmonary tuberculosis patients and elderly patients, chemotherapy combined with immunotherapy were more conducive to the rehabilitation of pulmonary tuberculosis patients whose expression levels of immune cells were significantly reduced.

Key words： tuberculosis pulmonary humoral immunity cellular immunity

Received: 07 November 2017

PACS:

R521

Corresponding Authors: Hou Yuan-pei,Email:hhyyppxz@163.com

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	ZHU Chun-ling
	HOU Yuan-pei
	YOU Lian
	LIU Cheng-yong

Cite this article:

ZHU Chun-ling,HOU Yuan-pei,YOU Lian等. Expression level and clinical significance of immune cells in peripheral blood of patients with pulmonary tuberculosis[J]. Chinese Journal of Zoonoses, 2018, 34(7): 648-652.

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http://www.rsghb.cn/EN/10.3969/j.issn.1002-2694.2018.00.106 OR http://www.rsghb.cn/EN/Y2018/V34/I7/648

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