海南1例感染类鼻疽伯克霍尔德菌的溯源调查

doi:10.3969/j.issn.1002-2694.2018.00.111

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Abstract We aimed to identify the infectious source of a case of melioidosis and to provide evidence for the prevention and control of melioidosis in Hainan Province, China. Environmental sampling was carried out in the surrounding areas of the patient’s residence to perform the culture-based etiological survey. Suspected strains of Burkholderia pseudomallei were isolated using Ashdown selective plates and further confirmed by bacteriological tests, 16S rDNA sequencing, and specific diagnostic PCR assays. Environmental and clinical isolates of B. pseudomallei were characterized by pulsed field gel electrophoresis (PFGE), multi-locus sequence typing (MLST) and multi-locus variable tandem repeat analysis (MLVA), and then the homology and the genetic relationships between them were analyzed. Results showed that a total of 58 soil or water samples were collected in this study. Three strains of B. pseudomallei were isolated in three specimens of domestic well water, among which 2 from the patient’s house and 1 from the neighbor’s house respectively. One of these environmental isolates was identical to the clinical isolate of the patient by MLST, PFGE, and MLVA, indicating well water being the source of infection for this case of melioidosis. Domestic well waters at the melioidosis patient’s residence and the neighbor’s residence had been contaminated by B. pseudomallei, and it is probable that well water be the source of infection for this patient.

Key words： melioidosis Burkholderia pseudomallei molecular tracking

Received: 20 November 2017

PACS:

R379.9

Fund:Supported by grants from the National Natural Science Foundation of China (No.81573208), the Key Research Development, Scientific and Technological Cooperation Project of Hainan Province (No. ZDYF2017163), and the Natural Science Foundation of Hainan Province (Nos. 814389 & 817398)

Corresponding Authors: Zheng Xiao, Email: zhengxiao@icdc.cn; Zhu Xiong, Email: zhuxiong6@163.com

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	LI Sha
	CHEN Hai
	LI Huan
	LI Wei
	XIA Lian-xu
	LI Li-da
	ZHENG Xiao
	ZHU Xiong

Cite this article:

LI Sha,CHEN Hai,LI Huan等. Molecular tracing investigation for one case of melioidosis in Hainan Island, China[J]. Chinese Journal of Zoonoses, 2018, 34(7): 673-676.

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http://www.rsghb.cn/EN/10.3969/j.issn.1002-2694.2018.00.111 OR http://www.rsghb.cn/EN/Y2018/V34/I7/673

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