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Identification and genetic characteristics of non-polio enteroviruses isolated from acute flaccid paralysis cases in Guizhou Province of China,2013 |
WANG Yu, REN Gang, WANG Yin-yin, SU Fei, TANG Xiao-min, YE Xu-fang |
Guizhou Center for Disease Control and Prevention, Guiyang 550004, China |
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Abstract To study the genotype of non-polio enterovirus (NPEV) isolates from acute flaccid paralysis (AFP) cases in Guizhou Province of China in 2013 and to analyze the genetic characteristics of coxsackievirus A4 (CVA4) strains, a total of 235 cases under 15 years old with AFP were reported of Guizhou Province of China. Human rhabdomyosarcoma (RD) cells and L20B cells were used for virus isolation. The serotypes of isolated NPEV strains were identified by VP1 sequencing, phylogenetic tree was constructed by MEGA7.0 software for genetic characterization based on neighbor joining algorithm and the reliability of the phylogenetic trees was determined by bootstrap analysis with 1 000 pseudo replicate datasets. The results showed that 24 NPEV strains were isolates from 235 AFP cases, including 2 strains un-typed. Among the 22 strains, a total of 12 serotypes were identified, 6 strains belonged to human enterovires (HEV) A viruses including 4 serotypes, 3 strains was CVA 4 and belonged to the C2 subgenotype. A total of 15 strains belonged to HEV-B viruses including 7 serotypes and the other 1 strains belonged to HEV-C group. No NPEV strain of HEV-D group was detected. In 2013, the pathogen spectrum of AFP cases included several serotypes of HEV and the genetic characteristics showed that CVA4 strains of C2 subgenotype were the predominant genotype in Guizhou Province.
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Received: 21 September 2017
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Fund:Supported by the Guizhou Health and Family Commission Science Foundation (No. gzwjk2016-1-054) |
Corresponding Authors:
Ye Xu-fang, Email: 496108556@qq.com
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