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| Comparison of neurovirulence and full-length sequence of two rabies virus clones with different virulence |
| SHI Lei-tai, LI Yu-hua, YU Yong-xin |
| Arboviral Vaccine Division, National Institutes for Food and Drug Control, Beijing102629, China |
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Abstract We aimed to compare the neurovirulence and genome sequence differences between two rabies virus clones with different virulence. The different ages(10 d, 14 d and 21 d) of mice were tested for the neurovirulence(LD50) of the two clones by intracerebral inoculation. The inoculated virus content(PFU) was tested by plaque assay. The lgPFU/lgLD50 values of the two clones were calculated and compared. The full-length genomes of the two clones were sequenced and compared. The results showed the values of lgPFU/lgLD50 tested in the 10 d, 14 d, and 21 d-old mice with CTN181-3 strain were higher (0.07, 3.40 and 6.60, respectively) than that of the CTN181-12 strain(<-0.9, <-0.6 and 1.04, respectively), which indicated the neurovirulence of CTN181-3 strain was lower than that of CTN181-12 strain. The full-length sequence analysis showed that there were eight nucleotides and six amino acids differences between the two virus clones. The neurovirulence of CTN181-3 is lower than that of CTN181-12 strain and the six amino acids difference between them are the molecular basis for the neurovirulence.
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Received: 22 February 2018
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Corresponding Authors:
Yu Yong-xin, Email: yuyongxin@nicpbp.org.cn
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2018, Vol. 34 
