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Serogroup distribution and characteristics of molecular subtyping for Neisseria meningitidis isolated from Jiangxi, 2005-2015 |
YANG Meng1, ZHOU Hai-jian2, XU Xiao-qian1, XIONG Chang-hui1, WANG Peng1, LIU Xiao-qing1, YUAN Hui1 |
1. Department of Communicable Disease Control and Prevention, Jiangxi Province Center for Disease Control and Prevention, Nanchang 330029, China; 2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing 102206, China |
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Abstract We analyzed the serogroup distribution and molecular subtyping characteristics of Neisseria meningitidis isolated from Jiangxi during 2005 and 2015, and revealed the characteristics and change of population structure. A total of 200 N.meningitides isolated during 2005 and 2015 were investigated by serogroup, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Clustering tree and minimum spanning tree was constructed by using BioNumerics software. The distribution of serogroup and molecular subtypes isolated in different sources and years were analyzed. Among the 200 N. meningitidis, the serogroup were C (101), B (50), A (12), W135 (2), Y (2), X (1), non-groupable (32). There were 100%, 86.0% and 28.9% of the clinical, close contact and healthy carried isolates were serogroup C. Among healthy carried isolates, there were 1.9%, 58.8%, 81.5% and 0% of the strains isolated from 2005, 2007, 2008 and 2012 were serogroup C. The 95 serogroup C strains were isolated into 14 PFGE types and among them NMNh.JX0001 and NMNh.JX0002 were dominant types, including 46 and 30 strains respectively. Among the serogroup C strains isolated during 2005-2006, 2007-2008 and 2013-2014, proportion of NMNh.JX0001 strains were 97.2%, 28.6% and 10.0%respectively, and proportion of NMNh.JX0002 strains were 2.8%, 57.1%, 90.0%. Thirty-three serogroup C strains with dominant PFGE types were analyzed by MLST and all were ST-4821. All the meningococcal diseases in Jiangxi between 2005 and 2015 were caused by serogroup C strains. Serogroup C strains have also existed in healthy population. Although the dominant ST of serogroup C has not changed in the past 10 years, the PFGE types have been changed, suggesting that the genome of bacteria exists microevolution. This microevolution might cause the change of epidemic model. Continuous monitoring is necessary.
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Received: 26 October 2017
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Fund:Supported by the Priority Project on Infectious Disease Control and Prevention (No. 2013zx10004203-002-007) from the Ministry of Health, China, and the Health and Family Planning Commission of Jiangxi Province (No. 20062004) |
Corresponding Authors:
Yuan Hui, Email: ymss921@126.com
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