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Complete genome analysis of human enterovirus 71 strain isolated in Nanjing,China |
WANG Zi-ming1,YU Qian1,ZHANG Zeng-di2,HUANG Zan1 |
1. Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China; 2. Nanjing Children’s Hospital, Nanjing 210008, China |
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Abstract To provide a basis for monitoring and control of HFMD caused by enterovirus 71 in Nanjing 2017, we isolated two EV71 strains from clinical EV71-RNA positive fecal samples. The evolution and genetic variation characteristics of these two strains were determined by their whole genomic nucleotide sequence. To this end, we fractionally amplified the genomes by PCR with 8 pairs of primers, and sequenced these amplicons to splice the whole genome. The nucleotide and amino acid homologous comparison and phylogenetic analysis were conducted with other reference strains. Homology analysis of genomic nucleotide showed that the homology of these two isolates was 94%. With EV71 NJ2017iso2 as a reference, it had the highest homology with the strain Fuyang 17.08-02 (96.9%), but lower homology with the prototype strain BrCr (79.8%). Meanwhile, phylogenetic trees based on VP1 gene or genome sequences indicated that NJ2017 isolates were clustered with references strain of C4 subgenotype and there was no major mutation compared with other previously reported C4 epidemic strains in China. Therefore, these two newly isolated EV71 strains belong to the C4a type. Although their nucleotide sequences were significantly different from each other, most variations were nonsense mutations and their corresponding amino acid sequences were relatively stable.
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Received: 08 December 2017
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Fund:Supported by grands from the National Natural Science Foundation of China (No.31500944) and the Natural Science Foundation in Jiangsu Province (Nos.BK20150687, BK20170147) |
Corresponding Authors:
Zan Huang, Email: huangzan@njau.edu.cn
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