一株猴源空肠弯曲菌的鉴定及其生物学特性分析

doi:10.3969/j.issn.1002-2694.2018.00.205

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Abstract Campylobacter jejuni (C. jejuni) is one of the most common foodborne pathogens causing diarrhea, especially in the developed countries. However, the pathogenic mechanism of C. jejuni remains uncertain by now. The variation of the sequenced type strain NCTC1168 seems to be universal, bothering researchers to some extent. A strain named as M166, which was isolated from the anal swabs of a diarrhea Macaca with suspected C. jejuni infection, was confirmed as C. jejuni by multiple PCR, VITEK2 compact microbial identification system test, sodium hippurate hydrolysis experiment, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) test and electron microscope observation. Compared with NCTC11168, though the growth rate and proliferation of M166 in MH were not as good as that of NCTC11168, the motility of M166 was much more active than NCTC11168. M166-inoculated Balb/c mice carried C.jejuni in their feces constantly, and the loads of C.jejuni were detected in their duodenum, jejunum, ileum and cecum with 3.09±0.47, 3.01±0.84, 3.80±0.46, 5.63±0.34, respectively, while NCTC11168 wasn’t detected 7 days post-inoculation, which indicates that M166 has better colonization ability in intestinal tracts. Our study has observed that strain M166 is able to actively colonize in mice, which indicates its pathogenicity in Macaca, and a further research is required to evaluate the pathogenicity of this strain.

Key words： Campylobacter biochemical identification MALDI-TOF-MS biological characteristics colonization Macaca-source

Received: 14 March 2018

PACS:

R378.99

Fund:Supported by the Key Project of National Science and Technology Support Plan of China (No. 2014BAD13B02), the Key Research and Development Program (Modern Agriculture) Project of Jiangsu Province (No. BE2017341), and the Jiangsu Province Agricultural Science and Technology Independent Innovation Funds [No. CX(16)1028]

Corresponding Authors: Huang Jin-lin, Email: jinlin@yzu.edu.cn

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	JIN Wei-wei
	TANG Hai-yan
	REN Fang-zhe
	TANG Hong
	JIAO Xin-an
	HUANG Jin-lin

Cite this article:

JIN Wei-wei,TANG Hai-yan,REN Fang-zhe等. Identification and biological characterization of a Macaca-source Campylobacter jejuni strain[J]. Chinese Journal of Zoonoses, 2018, 34(12): 1102-1106.

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http://www.rsghb.cn/EN/10.3969/j.issn.1002-2694.2018.00.205 OR http://www.rsghb.cn/EN/Y2018/V34/I12/1102

[1] Manasson J, Shen N, Ferrer H, et al. Gut microbiota perturbations in reactive arthritis and postinfectious spondyloarthritis[J]. Arthritis & rheum, 2018, 70(2): 242-254. DOI:10.1002/art.40359
[2] Azimi T, Nasiri MJ, Chirani AS, et al. The role of bacteria in the inflammatory bowel disease development: a narrative review[J]. Acta Pathol Microbiol Scand, 2018,126(4):275-283. DOI: 10.1111/apm.12814
[3] Russell RG, Blaser MJ, Sarmiento JI, et al. Experimental Campylobacter jejuni infection in Macaca nemestrina[J]. Infect Immun, 1989, 57(5): 1438-1444.
[4] Silva J, Leite D, Fernandes M, et al. Campylobacter spp. as a foodborne pathogen: a review[J]. Front Microbiol, 2011, 2(1): 200. DOI: 10.3389/fmicb.2011.00200
[5] 周勤,郭光远.实验猴弯曲菌感染情况调查研究[J].云南大学学报(自然科学版),2006(S1): 371-373,376.
[6] Koga T, Aoki W, Mizuno T, et al. Antimicrobial resistance in Campylobacter coli and Campylobacter jejuni in cynomolgus monkeys (Macaca fascicularis) and eradication regimens[J]. J Microbiol Immunol Infect, 2017, 50(1): 75-82.
[7] 何蕊,黄金林,许海燕,等.弯曲菌多重PCR检测方法的建立及其初步应用[J].扬州大学学报(农业与生命科学版), 2007, 28(1): 5-8.
[8] Shang YW, Ren FZ, Song ZJ, et al. Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model[J]. Sci Rep, 2016, 6: 28737. DOI: 10.1038/srep28737
[9] Martiny D, Dediste A, Debruyne L, et al. Accuracy of the API Campy system, the Vitek 2 Neisseria-Haemophilus, card and matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of Campylobacter, and related organisms[J]. Clin Microbiol Infect, 2011, 17(7): 1001-1006. DOI: 10.1111/j.1469-0691.2010.03328.x
[10] Soutourina OA, Bertin PN. Regulation cascade of flagellar expression in Gram-negative bacteria[J]. FEMS Microbiol Rev, 2003, 27(4): 505-523.
[11] Frirdich E, Biboy J, Adams C, et al.Peptidoglycan-modifying enzyme Pgp1 is required for helical cell shape and pathogenicity traits in Campylobacter jejuni[J]. PLoS Pathog, 2012, 8(3): e1002602. DOI: 10.1371/journal.ppat.1002602
[12] Frirdich E, Vermeulen J, Biboy J, et al. Peptidoglycan LD-carboxypeptidase Pgp2 influences Campylobacter jejuni helical cell shape and pathogenic properties and provides the substrate for the DL-carboxypeptidase Pgp1[J]. J Biol Chem, 2014, 289(12): 8007-8018.
[13] Esson D, Mather AE, Scanlan E, et al. Genomic variations leading to alterations in cell morphology of Campylobacter spp[J]. Sci Reports, 2016, 6: 38303. DOI: 10.1038/srep38303
[14] Parkhill J, Wren B W, Mungall K, et al. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences[J]. Nature, 2000, 403(6770): 665-668. DOI:10.1038/35001088
[15] Walter F, Ott J, Claus H, et al. Sex- and age patterns in incidence of infectious diseases in Germany: Analyses of surveillance records over a 13-year period (2001－2013) [J]. Epidemiol Infect, 2018: 1-7. DOI:10.1017/S0950268817002771
[16] Kuhn KG, Nielsen EM, Molbak K, et al. Epidemiology of campylobacteriosis in Denmark 2000-2015[J]. Zoonoses Public Health, 2018, 146(3): 372-378.
[17] Cawthraw SA, Wassenaar TM, Ayling R, et al. Increased colonization potential of Campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks[J]. Epidemiol Infect, 1996, 117(1): 213-215.
[18] Gaynor EC, Cawthraw S, Manning G, et al. The genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes[J]. J Bacteriol, 2004, 186(2): 503-517.