|
|
Genotyping and drug resistance of 127 strains of Mycobacterium tuberculosis |
LIU Yi-ping1, PANG Hui2, WAN Kang-lin3, GONG Yan-e4, GUO Xu-xia4, WANG Yan-xia4, LIU Hai-can3 |
1. Department of Medical Parasitology, Changzhi Medical College, Changzhi 046000, China; 2. Department of Immunology, Changzhi Medical College, Changzhi 046000, China; 3. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 4. Department of Inspection, Heping Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China |
|
|
Abstract To understand the genetic polymorphism and the correlation of genotype and drug resistance of Mycobacterium tuberculosis (M. tuberculosis) circulating in Changzhi and the surrounding area in Shanxi Province, multi-loci variable number tandem repeats (VNTRs) analysis (MLVA) were utilized for the genotyping of the clinical isolates and drug susceptibility test (DST) of the 8 antituberculosis agents was performed on the Lowenstein-Jensen (L-J) medium by the proportion method. The BioNumerics (Version 5.0) software was used for clustering analysis. The result showed that Mtub21, MIRU26 and ETRE revealed high polymorphism (Hunter-Gaston Index, HGI>0.6). MIRU40, ETRB, MIRU16, ETRD, MIRU23 and ETRC showed low polymorphism (HGI<0.3). The 127 M. tuberculosis strains were divided into 11 groups of which the groupⅠ accounting for the largest percentage of 80.31% and 102 genotypes including 90 idiotopes. The drug resistance of the first-line antituberculosis agents and the second-line antituberculosis drugs were 47.24% and 23.62%. Among the first-line antituberculosis agents, streptomycin (SM) (40.94%) had the highest drug resistance, and ethambutol (EMB) (6.30%) was the best antibacterial agents against experimental strains. Of the second-line antituberculosis drugs, protionamide (PTH) (3.15%) was the good choice with the lowest drug resistance, and levofloxacin (LFX) (14.17%) had the highest drug resistance. The single drug resistance of streptomycin (11.81%) was the highest followed by isoniazid (2.36%), and we found that no strains were drug resistant for single rifampicin or single ethambutol. The 21.26% (27/127) were multi-drug resistant, and the 3.15% were widely multi-drug resistant. It’s no statistical significance that the difference of the drug resistance rate between the groupⅠ strains and other strains. In conclusion, it’s preliminarily confirmed that the M. tuberculosis has obvious genetic polymorphism and the group Ⅰ strains were popular in Changzhi and the surrounding area. The sensitivity to the second-line antituberculosis drugs was higher than that to the first-line antituberculosis drugs. It had no obvious correlation between the major epidemic strains groupⅠand drug resistance.
|
Received: 06 February 2018
|
|
Fund:Supported by the Science Foundation for Youths of Shanxi Province (No. 2016021161), the Shanxi Education Science "Thirteenth Five" Planning Issue (No. GH17047), the Innovation and Training Program for College Students of Shanxi Province (No. 2016303), the Innovation and Training Program for College Students of Changzhi Medical college (No. D2017015), the Second Batch of Continuing Education Program in Shanxi Province (No. 130308197), and the Changzhi Medical College Science and Technology Innovation Team Support Project (No. CX201412) |
Corresponding Authors:
Pang Hui, Email: panghuimail@126.com
|
|
|
|
[1] WHO. Global tuberculosis report 2017 [EB/OL]. [2017-10-30]. http: // www. who.int/tb/ publications/ global _ report/zh/. [2] 曹晓慧,刘志广,赵秀芹,等. 220株结核分枝杆菌北京临床分离株的基因分型研究[J].中国人兽共患病学报, 2008, 24(5): 412-417. [3] 李道群,夏雪山,宋玉竹,等.结核分枝杆菌分子分型技术的研究和应用[J].中国人兽共患病学报, 2016,32(1): 76-82. DOI: 10. 3969 / j. issn. 1002-2694. 2016. 01. 016 [4] 李斌,刘海灿,王兆芬,等.青海省结核分枝杆菌临床分离株可变数目串联重复序列基因多态性研究[J].中华流行病学杂志,2015, 36(10): 1158-1161. DOI: 10. 3760 / cma. j. issn. 0254-6450. 2015. 10. 026 [5] Pang H, Tong J, Liu HC, et al. Molecular characterization and drug-resistance of Mycobacterium tuberculosis strains in Xuzhou, China[J]. Bio Environ Sci, 2014, 27(12): 960-964. DOI: 10. 3967 / bes. 2014. 136 [6] 李君莲,刘洁,鱼栓民,等.新疆结核分枝杆菌临床分离株VNTR基因型的初步研究[J]. 中国人兽共患病学报, 2010, 26(12): 1105-1109. [7] Liu J, Tong C, Liu J, et al. First insight into the genotypic diversity of clinical Mycobacterium tuberculosis isolates from Gansu Province, China[J]. PLoS One, 2014, 9(6): e99357. DOI: 10. 1371 / journal. pone. 0099357 [8] Clinical and Laboratory Standards Institute. Susceptibility testing of Mycobacteria, Nocardiae, and other aerobic actinomycetes; approved standard-second edition[S]. CLSI Document, 2011, M24-A2. [9] Liu Y, Tian M, Wang X, et al. Genotypic diversity analysis of Mycobacterium tuberculosis strains collected from Beijing in 2009, using spoligotyping and VNTR typing[J]. PLoS One, 2014, 9(9): e106787. DOI: 10. 1371 / journal. pone. 0106787 [10] 石荔,范昕建,万康林.多位点可变串联重复序列技术用于西藏地区结核分枝杆菌基因分型的初步研究[J].中华流行病学杂志, 2007, 28(5): 477-481. [11] 陈志,陈佳,段晋超,等.绵阳地区79株结核分枝杆菌的基因型特征研究[J].中华微生物学与免疫学杂志, 2011, 3(3): 200. DOI: 10. 3760 / cma. j. issn. 0254-5101. 2011. 03. 002 [12] Shamputa IC, Lee J, Allix-Béguec C, et al. Genetic diversity of Mycobacterium tuberculosis isolates from a tertiary care tuberculosis hospital in South Korea[J]. J Clin Microbiol, 2010, 48(2): 387-394. DOI: 10.1128 / JCM. 02167-09 [13] 蒋毅,张丽水,赵秀芹,等. MLVA技术用于福建105株结核分枝杆菌基因分型的初步研究[J].中国人兽共患病学报, 2007, 23(1): 1-4. [14] 李心德.174株耐多药结核分枝杆菌对二线抗结核药耐药情况分析[J].国际检验医学杂志2014,(13):1732-1733,1748. DOI: 10.3969 / j.issn.1673-4230. 2014. 13. 028 [15] 全国第五次结核病流行病学抽样调查技术指导组, 全国第五次结核病流行病学抽样调查办公室. 2010年全国第五次结核病流行病学抽样调查报告[J].中国防痨杂志, 2012, 34(8): 485-508. [16] 陈丽峰,周菁, 陈军,等.武汉市1561例结核患者的耐药性分析[J].临床肺科杂志,2014, 19(9): 1638-1640. DOI: 10. 3969 / j. issn. 1009-6663. 2014. 09. 028 [17] 季登英,张燕,马欣慧.左氧氟沙星辅助抗结核药物治疗耐多药肺结核的临床疗效[J].临床合理用药, 2015, 8(9): 14-15, 18. DOI: 10. 15887 / j. cnki. 13-1389 / r. 2015. 27. 007 [18] Li Y, Cao X, Li S, et al. Characterization of Mycobacterium tuberculosis isolates from Hebei, China: genotypes and drug susceptibility phenotypes[J]. BMC Infect Dis, 2016,16: 107. DOI: 10. 1186 / s12879-016-1441-2 [19] 庞慧, 李桂莲, 万康林, 等. 34株慢速生长非结核分枝杆菌针对一线和二线抗结核药物的敏感性试验研究[J]. 中国人兽共患病学报, 2015, 31(10): 914-918. DOI: 10. 3969 / j. issn. 1002-2694. 2015. 10. 004 [20] 赵丽芬, 刘延梅. 2013-2015年西安市胸科医院结核分枝杆菌的耐药性分析[J]. 现代药物与临床, 2015, 30(12): 1542-1545. DOI: 10.7501 / j. issn. 1674-5515. 2015. 12. 026 |
[1] |
WU Yi-fei, ZHAO Gang, CHEN Shu-dan, ZHANG Xue-chao, LI Qing-chun, LU Min, CHENG Qing-lin, YAN Jie, XIE Li. Pathogens and risk factors of pulmonary tuberculosis and predominant resistance spectra of Mycobacterium tuberculosis[J]. Chinese Journal of Zoonoses, 2019, 35(6): 480-486. |
|
|
|
|