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Prevalence and antimicrobial resistance analysis of Campylobacter spp. from broiler slaughter and processing chain |
TANG Meng-jun, ZHOU Qian, ZHANG Xiao-yan, ZHOU Sheng, ZHANG Jing, TANG Xiu-jun, GAO Yu-shi |
Jiangsu Institute of Poultry Science, Yangzhou 225125, China |
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Abstract The aim of this study is to investigate the contamination of Campylobacter spp. from broiler slaughter and processing in chicken chain and its antimicrobial resistance, the drug resistant genes in Jiangsu Province, China. A total of 170 samples were collected from each section during chicken slaughtering and processing. CCDA plate was selected for separation and purification. PCR was performed to identify Campylobacter species including Campylobacter jejuni (C. jejuni) and Campylobacter coli (C.coli). Susceptibility of bacteria to 14 antibiotics belong to 6 categories was determined by the Kirby-Bauer (K-B) method as recommended by the CLSI. The class Ⅰ integron was detected through the int1 gene amplification by PCR. Tet(O) and aadE-sat4-aphA-3 were found by PCR. The Campylobacter isolates of the point mutation of gyrA gene at DNA sequence position 257 was examined by PCR and sequencing. Results showed that 124 Campylobacter isolates were obtained from 170 samples (72.95%). The resistant rates for Campylobacter isolates were 100% against Streptomycin, Tobramycin, Kanamycin, Ciprofloxacin, Ofloxacin, Nalidixic acid, Erythromycin and Tetracycline. The multi-drug resistance was common among Campylobacter isolates and the multiple resistant rate was 100%. The regnant drug-resistant spectrum is CTX-CRO-cDA-K-NOR-S-AZM-CIP-TE-NA-E-LEV-ENR-TOB. The class Ⅰ integron of Campylobcter isolates did not detected. The sequencing results showed that all of the Campylobacter isolates had the Thr-86-Ile point mutation in gyrA at quinolone resistant determining region. The detection rate of tet(O) and aadE-sat4-aphA-3 gene cluster were 100% and 90.32%, respectively. This study provided that the Campylobacter contamination occurred in the broilers slaughter and processing chain, and the antimicrobial resistance of Campylobacter isolates was relatively serious. There was significant correlation between antimicrobial phenotypes of Campylobacter isolates with their gene.
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Received: 27 March 2018
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Fund:Supported by the National Natural Science Foundation of China (No. 31700005), the Modern Agro-Industry Technology Research System of Jiangsu Province [No. JATS(2017)256], and the Yangzhou Social Development Project (Nos. YZ2017079 and YZ2016058) |
Corresponding Authors:
Gao Yu-Shi, Email: 475002207@qq.com
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