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| Exploration of a rapid test strip for brucellosis |
| KONG Yu-fang1, ZHENG Jia-hao2, WANG Hui-yu1, MEI Lin1, WU Shao-qiang1, LIN Xiang-mei1, HAN Xue-qing1 |
1.Chinese Academy of Inspection and Quarantine,Beijing 100176,China;
2.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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Abstract To develop a simple, sensitive and specific method on detection of antibodies against Brucellosis, the recombinant Brucella outer membrane protein OMP22 and OMP28 was labeled with colloidal gold, anti-OMP22 monoclonal antibody was used to provide the control line, and OMP22 and OMP28 mixed protein was served as test line, respectively. The results showed that the maximum dilution of the Bovine Brucella standard positive serum was 1:128. There was no cross reaction with the sera of other related disease. The rate of concordance between strips and ELISA kit (IDEXX) was 95%, and the coincidence rate with the tiger red plate aggutination test(RBPT) was 97.4%. It is suggested that the colloidal gold immunochromatographic test strip can be used to quickly and accurately detect antibodies against brucellosis and to be suitable for clinical diagnosis and epidemiological study of brucellosis.
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Received: 15 February 2019
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| Fund:Supported by the National Key R&D Program of China(No.2016YFD0501106),Research Program of Chinese Academy of Inspection and Quarantine(No.2018JK012)Kong Yu-fang and Zheng Jia-hao have an equal contribution to this paper. |
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Corresponding Authors:
HAN Xue-qing,Email:xqhancaiq@163.com
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[1] Rossetti CA, Arenas-Gamboa AM, Maurizio E, et al.Caprine brucellosis:a historically neglecteddis ease with significant impact on public health[J].PLoS Negl Trop Dis,2017,11(8):e0005692. DOI:10.1371/journal.Dis, 2017,11(8):e0005692
[2] 毛景东,王景龙,杨艳玲.布鲁氏菌病的研究进展[J].中国畜牧兽医,2011, 38(1): 222-226.
[3] Zhao HJ, Tu YP, Sheng SF, et al.Advances in laboratory diagnostic techniques for Brucellosis[J]. Chin Ani Qua, 2013, 30(5):82-85.
[4] 张宇,王志坚.常规试管凝集试验与虎红平板凝集试验检测布病抗体结果对比分析[J].畜牧兽医,2018,6:222.
[5] Patra KP, Saito M, Atluri VL, et al.A Protein-conjugate approach to develop monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis[J].PLoS Negl Trop Dis,2014,8:e2926. DOI:10.1371 /journal.pntd.0002926
[6] Lindler LE, Hadfield TL, Tall BD, et al.Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, aprotein recognized by the humoral immune recognized by the humoral immune response during human brucellosis[J].Infect immun,1996,64(7):2490-2499.
[7] 熊流新, 黄健, 张涛,等. 布鲁氏菌外膜蛋白 Omp16的原核表达、纯化及多克隆抗体制备[J].现代医药卫生,2018,34(5):669-673. DOI:10.3969/j.issn.1009-5519.2018.05.008
[8] 宫晓炜,景涛,王治国,等.布氏杆菌BP26蛋白的表达纯化及其抗原性的研究[J].中国人兽共患病学报,2009,25(11):1085-1088.
[9] Kaushi KP, Singh DK, Kumar SV, et al.Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant OMP28 adjuvanted with CpG oligonucleotides[J].Vet res commun,2010,34(2):119-132. DOI: 10.1007/s11259-009-9337-x
[10] Cloeckaert A, Verger M, Grayon M, et al.Classification of Brucella spp. isolated from marine manuals by DNA polymorphism at the OMP22 locus[J]. Microbes infect,2001,3:729-738.
[11] Delvecchio VG, Kapatral V, Elzer P, et al.The genome of Brucella melitensis[J]. Vet Microbiol, 2002, 90(1/4):587.DOI:10.1128/MRA.01189-18
[12] Martín-Martín AI, Caro-Hernández P, Orduña A, et al.Importance of the Omp25/Omp31 family in the internalization and intracellular replication of virulent B. ovis in murine macrophages and HeLa cells[J]. Microbiol & Infect, 2008, 10(6):706-710. DOI:10.1016/j.micinf.2008.02.013
[13] Cloeckaert A, Vizcation N.Major outermembrane proteins of Brucella spp: Past ,present and future[J].Vet Microbiol,2002,90(1/4):229-247.
[14] 冯宇, 胡莉萍, 朱良全, 等. 蛋白质组学技术在布鲁氏菌病研究中的应用及发展[J].微生物学通报, 2018,45(1):191-196. DOI: 10.13344/j.microbiol.china.170535
[15] Koyuncu I, Kocyigit A, Ozer A, et al.Diagnostic potential of Brucella melitensis Revlnative Omp28 precursor in human brucellosis[J].Cent Eur J Immunol,2018,43(1):81-89. DOI:10.5114/ceji.2018.74877
[16] Khan MZ, Zahoor M.An overview of brucellosis in cattle and humans, and its serological and molecular diagnosis in control strategies.[J]. Trop Med Infect Dis,2018,3(2) 283-289.DOI:10.3390 /tropicalmed 3020065
[17] Lim JJ, Kim DH, Lee JJ, et al.Protective effects of recombinant Brucella aboetus OMP28 against infection with a virulent strain of Brucella abortus 544 in mice[J].J Vet Sci,2012,13(3): 287-292. DOI:10.4142/jvs.2012.13.3.287
[18] TN A, Ashu K, Tiwari S, et al.Fed batch fermentation and purification stategy for high yield production of Brucella melitensis recombinant Omp28 kDa protein and its application in disease diagnosis[J]. Protein Peptide Lett,2013,20(7):731-740.
[19] Cassataro J,Pasquevich K, Bruno L, et al. Antibody reactivity to Omp31 from Brucella melitensis in human and animal infection by smooth and rough Brucellae[J].Clin Diagn Lab Immunol,2004,11(1):111-114. DOI:10.1128/CDLI.11.1.111-114.2004
[20] Alghoribi MF, Zidan KH, Alswaji AA, et al. Whole-genome sequence of a Brucella melitensis strain isolated from sheep in saudi Arabia[J]. Microbiol Resour Announc,2018,7(21):e01189-18. DOI:10.1128/MRA.01189-18
[21] Martín-Martín AI,Caro Hemández P, Orduna A, et al. Importance of the Omp25/Omp31 family in the internalization and intracellular replication of virulent B.ovis in murine macrophages and HeLa cells[J].Microbes Infec,2008,10:706-710. DOI:10.1016/j.micinf.2008.02.013
[22] Martín-Martín AI,Caro Hemández P, Sancho P, et al.Analysis of the occurence and ditribution of the Omp 25/Omp31 family of surface proteins in the six classical Brucella species[J].Vet Microbiol,2009,137(1/2):74-82. DOI:10.1016/j.vetmic.2008.12.003
[23] Edmonds D,Cloeckaert A, Hagius D,et al.Pathogenicity and protective activity in pregnant goats of a Brucella melitensis delta Omp25 deletion mutant[J].Res Vet Sci,2002,72:235-239.
[24] 刘亚东,王慧煜,刘佳佳,等.基于牛源布鲁氏杆菌重组膜蛋白的胶体金免疫层析试纸条的研制[J].中国兽医科学,2018,48(7):805-810. DOI:10.16656/j.issn.1673-4696.2018.0100 |
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2019, Vol. 35 
