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| Transmission pattern investigation of Brucella melitensis from Inner Mongolia, China |
| LIU Zhi-guo1, 2, WANG Miao3, ZHAO Hong-yan1, PIAO Dong-ri1, CUI Bu-yun1, LI Zhen-jun1 |
1. National Institute of Infectious Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Inner Mongolia Autonomous Region Central for Comprehensive Disease Control and Prevention, Huhhot 010031, China;
3.Ulanqab Center for Endemic Disease Control and Prevention, Ulanqab 012000, China |
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Abstract Aim of this study is revealed transmission pattern and epidemic feature of Brucella melitensis from Inner Mongolia, China. A total of 60 strains of Brucella were investigated and type of these Brucella strains were identified by AMOS-PCR. HIGD was used to assessed genetic diversity characteristics of strains in this study, MLVA was applied for gene-typing and determined genetic relationship between strains. AMOS-PCR confirmed that all of the testing strains belongs to B.melitensis, result showing that MLVA-16 exhibited the higher discriminating and diversity index was 0.981, diversity index of Panel 1, Panel 2A and Panel 2B were 0.264, 0.345 and 0.980, respectively, and bru16 in Panel 2B present the highest polymorphis in this assay, and diversity index was 0.835. A total of 60 strains were divided into 5 subgroups and comprised of 37 gene-types, which of 15 were shared gene-types in 38 strains including, with a clustering rates of 63.6% (38/60), these data showed that brucellosis exhibited multi-point outbreak in this region. Other 22 strains present unique gene-types that indicated these brucellosis was sporadic or without epidemiological link cases. GT5 gene-types were comprised of two strains obtained from sheep and camel, respectively, and had identical MLVA -16 gene-types that revealed B. melitensis were cycle transmission between sheep and camels. Three genetypes (GT11, GT17 and GT23) showing the same genetypes shared feature which strains were obtained from human and sheep exhibited the identical MLVA-16 gentypes, this data suggested that infected sheep was source of infection for human brucellosis.Three strains of GT35 were obtained from sheep spleen and shared identical MLVA -16 genetypes, hinted that sheep brucellosis exhibited outbreak. Twelve strains of E subgroup were isolated from different host (including in sheep, cattle, wild camel and human) and shared the identical or similarly MLVA-16 genetypes, this information maybe revealed a potential transmission pattern of B.melitensis from Inner Mongolia. In conclusion, infected sheep is source of infection for human brucellosis, camel or other wild animals maybe play as a role of reservoirs. B.melitensis are circulated transmission between sheep (cattle) and camels (other wild animals), later transmitted to humans is a potential transmission pattern of B.melitensis in examined area.
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Received: 08 August 2018
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| Fund:Supported by Nation Infectious Importance Special Fund (No. 2017ZX10303401), Bio-Safety Key Special Fund (No. 2017YFC1200303, No. 2016YFC1200701), Nature Science Fund of Inner Mongolia Autonomy Region (No. 2018MS08004) |
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Corresponding Authors:
Li Zhen-jun, Emial: LiZhenjun@icdc.cn
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2019, Vol. 35 
