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| Proteomic analysis of early subcutaneous parasitic Echinococcus multilocularis in mice |
| LAN Tian-yan1,2,ZHANG Yong-e2,GUO Peng-hui1,GUO Xiao-la2, CHEN Yi-xia1,WANG Ming-ming1,ZHENG Ya-dong2 |
1. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China;
2.State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
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Abstract To understand the proteomic characteristics of subcutaneous parasitic Echinococcus multilocularis. Approximately 600 protoscoleces were subcutaneously injected in each BALB/c mice. Subcutaneous parasitic hydatid cysts were isolated after one month. Then the tissue sections were prepared, and HE staining, glycogen staining, and morphological observation by microscope were conducted. Moreover, the proteome of hydatid cysts was determined, and then GO gene function analysis and COG analysis were performed. Finally, some proteins were verified by Western blotting. The subcutaneous parasitic Echinococcus multilocularis was transparent with a size of mung beans, which contained cystic fluid. Under the light microscope, mother capsules contained offspring cysts, and there were only a few protoscoleces, some of which had small hooks. Moreover, the glycogen staining was positive and the cyst wall was in pink. A total of 203 proteins were identified by sequencing, and the expression levels of various proteases were very high, such as phosphoenolpyruvate carboxykinase, citrate synthase and so forth. Functionally, the identified proteins are mainly enriched in post-translational modifications, protein turnover and chaperone signaling pathways, energy synthesis and conversion, and carbohydrate transport and metabolism, while proteins involved in immune responses, lipid transport, and metabolism were less. Among the top 20 proteins with high abundance, most proteins were involved in the process of glucose metabolism. Western blotting results showed that VCP, Enolase, 14-3-3 and β-actin were expressed in different degrees in the cyst, while GST was not expressed or expressed at a very low level, which was consistent with the sequencing results. The results suggested that subcutaneous parasitic Echinococcus multilocularis may have significant changes in glucose metabolism and fat metabolism, which provides valuable clues for exploring the mechanism of hydatid cyst infertility.
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Received: 02 September 2019
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Corresponding Authors:
Wang Ming-ming, Email: tracy4150@163.com
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2020, Vol. 36 
