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Association between HLA-B rs3493313 gene polymorphism and hemorrhagic fever with renal syndrome in southern Anhui Province |
LIU Gan1, HU Ting-ting1, TAO Dong-dong1, LIU Fa-hu2, YANG Jin-sun3, SUN En-tao1 |
1.School of Laboratory Medicine,Wannan Medical College,Wuhu 241002,China; 2.Department of Clinical Laboratory,Affiliated Yijishan Hospital of Wannan Medical College,Wuhu 241001,China; 3.Department of Infectious Diseases,Affiliated Yijishan Hospital of Wannan Medical College,Wuhu 241001,China |
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Abstract To investigate the relationship between single nucleotide polymorphisms (SNP) rs34933313 and Hemorrhagic fever with renal syndrome (HFRS) in the Han population of Southern Anhui Province. In this experiment, fresh blood samples from 14 HFRS patients (Case group) and 50 healthy patients (Control group) were collected and genomic DNA were extracted. HLA-B loci were classified by allele-specific primers polymerase chain reaction (ASP-PCR). The differences in alleles and genotypes between the two groups were in accordance with controlled by SPSS 22.0. The results showed that the distribution of rs34933313 of HLA-B gene in the groups was flowing Hardy Weinberg (H-W) genetic balance, and the differences of allele G、C(χ2=4.38,OR=2.45,P=0.04) and genotype GG、GC、CC(χ2=6.47,P=0.04) frequency distribution between the two groups were statistically significant, The distribution frequency of C allele in the case group was higher than that of G allele, and the probability of HFRS with C allele was G allele. The results of subgroup analysis showed that the rs34933313 locus of HLA-B gene was associated with the risk of HFRS in the dominant genetic model (χ2=6.45, P=0.01, OR=10.21, 95%CI=1.24~84.18). It is suggested that the rs34933313 locus of HLA-B gene is related to the risk of HFRS in the Han population of Southern Anhui Province, and the risk of HFRS will be increased in C allele.
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Received: 07 November 2019
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Fund:Supported by the National Natural Science Foundation of China (No. 31870352);The Youth Talent Support of Wannan Medical College(No.Wyqnyx201902);Research Fund for College Students of Wannan Medical College(No.wk2019s19);Innovation and Entrepreneurship Training Project for College Students in Anhui Province (No.201810368140) |
Corresponding Authors:
Sun En-tao, Email:asdentao@126.com;Yang Jin-sun, Email:yangjinsun999999@163.com
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