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Application for identification of Murine-like animals by DNA barcoding in Shaanxi Province |
CHEN Bao-bao, SUN Yang-xin, FAN Suo-ping, LYU Wen, NIE Shou-ming, LI Shen-zheng, AN Cui-hong |
Shaanxi Provincial Center for Disease Control and Prevention,Xi’an 710054,China |
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Abstract To explore the feasibility of DNA barcode technology for molecular biological identification and make up for the defects of morphological identification. Combined with plague monitoring work, rat-shaped animals were collected by sampling method,5-meter cage and night trapping method. After preliminary morphological identification of all specimens, molecular identification was carried out based on the mitochondrial cytochrome C oxidase I subunit gene (COI gene).And then the genetic distance was calculated and Neighbor-Joining tree was constructed. The COI gene sequences of 709 mouse-shaped animals were obtained, belonging to 38 species of 24 generas, 10 families and 3 orders. The COI gene sequences showed that the intra-species genetic distance was within 3.6%, with an average of 1.4%, the inter-species genetic distance was greater than 5.6%, the inter-species genetic distance was significantly greater than the intra-species genetic distance, and the boundaries between genera and families were not obvious. The morphological identification results of several mouse-shaped animals were corrected. Neighbor-Joining tree showed that individuals of the same species were clustered into a single branch with high support, and two cryptic species(C.griseus and C.sokolovi) were found. DNA barcoding technology can effectively identify rat-like animals, but due to the imperfect database, traditional taxonomic identification methods and modern molecular biological identification methods still need to be combined to ensure the accuracy of identification results.
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Received: 17 October 2019
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Fund:Supported by the Science and Technology Research and Development Program of Shaanxi Province (No. 2012K16-12-03) |
Corresponding Authors:
An Cui-hong,Email:an-ch@163.com
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