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| Effect of resveratrol against Echinococcus multilocularis protoscoleces and microcysts in vitro |
| GAO Hai-jun1,2,3,4, PANG Hua-sheng1, ZHANG Yan2, Huo Le-le3, JIANG Bin3, MO Xiao-jin3, LUO Yan-ping2, ZHANG Ting1,3, JING Tao2, XU Bin3, MA Xing-ming2, HU Wei3 |
1. Tibet Autonomous Region Center for Disease Control and Prevention, National Health Commission Key Laboratory of Echinococcosis Prevention and Control, Lhasa 850000, China;
2. School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China;
3. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); NHC Key Laboratory of Parasite and Vector Biology; WHO Collaborating Center for Tropical Diseases; National Center for International Research on Tropical Diseases, Shanghai 200025, China;
4. Garzr Tibetan Autonomous Prefecture Center for Disease Control and Prevention, Kangding 626000, China |
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Abstract To investigate the effect of resveratrol (RES) against protoscoleces (PSCs) and microcysts of Echinococcus multilocularis in vitro, providing the new references for drugs application to treat alveolar echinococcosis. The vitality and morphological changes in PSCs exposed to RES at different concentrations (5, 10, 20, 40, 80, 100, 200 or 400 μmol/L) for 7 days in vitro were investigated by staining with 0.4% trypan blue, and the ultrastructural alterations were imaged by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively; alkaline phosphatase (ALP) activity in culture supernatants was measured using chemiluminescence method. Further, the viability and morphology of E. multilocularis microcysts after treatment with RES for 14 days in vitro were investigated under a light microscopy, and then ALP activity in culture supernatant was measured using chemiluminescence method. The mortality of E. multilocularis PSCs increased with a dose- and time-dependent manner after treatment with RES at 5 to 400 μmol/L during 7 days in vitro, accompanying with obvious hooks loss. The mortality of PSCs reached 100% after treatment with 40 μmol/L RES for 6 days, and the ultrastructure of suckers, hooks and tegument were destroyed, however, under the same conditions, the mortality of PSCs was only (13.25±1.41)% and (6±0.71)% after treatment with ABZ-SO and dimethye sulfoxide (DMSO), respectively. The ALP activity in culture supernatant of PSCs significantly increased with a dose-dependent manner (F=36.94, P<0.001) on 6th day at different concentrations of RES. When treated with 20 or 40 μmol/L RES, the ALP activity was (2.72±0.24) U/L and (2.95±0.10) U/L, respectively, which posed significant differences in comparison with DMSO treatment (1.97±0.18) U/L (t=2.94, P=0.026 and t=7.066, P=0.000). In addition, after treatment with 40 μmol/L RES for 14 days, light transmittance in E. multilocularis microcysts deceased observably, and the germinal layer was shrunk and separated from laminated layer. Meanwhile, ALP activity (2.74±0.15) U/L in RES group was observably higher than that in DMSO group (2.08±0.09) U/L (t=3.83, P=0.019), but there was no significant difference between ABZ-SO group (2.29±0.14) U/L and DMSO group (t=1.271, P=0.273). Resveratrol could obviously kill E. multilocularis PSCs and microcysts in vitro, which may be a potential candidate drug for treatment of alveolar echinococcosis.
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Received: 17 March 2021
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| Fund:Supported by the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (No. 2019PT320004), the National Natural Science Foundation of China (No. 31860697,No. 81171632 and No. 81201315), and the Foundation of Shanghai Municipal Health Commission (No. 201840133). Gao Hai-jun and Pang Hua-sheng have the same contributions to the article. |
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Corresponding Authors:
Zhang Ting, Email: zhangting@nipd.chinacdc.cn; Jing Tao, Email: landa021001@126.com
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2021, Vol. 37 
