重庆綦江赶水地区库蠓及其胃血血源分子鉴定

doi:10.3969/j.issn.1002-2694.2021.00.155

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1593 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract This study aimed to use the mitochondrial CO Ⅰ gene of the species to detect the source of gastric blood from the blood-sucking Culicoides, and to study the blood-sucking habits of the midges. The mosquito trap method was used to collect blood-sucking Culicoides in different habitats in the in the Ganshui Town, Qijiang District, Chongqing, China, and to investigate the species composition of blood-sucking Culicoides in the area; at the same time, according to the differences in the mitochondrial CO Ⅰ gene sequence of the possible blood-sucking objects of the blood-sucking objects in the surrounding environment, the species-specific design was designed Using PCR technology to amplify the mitochondrial CO Ⅰ gene of blood meal of blood-sucking midge stomach, by matching the amplified product with the target band size corresponding to different species, detect the source of blood meal of blood-sucking Culicoides stomach. The results showed that the habitats of chicken houses, sheep houses and chicken-duck mixed houses were mainly C. arakawai, accounting for 93.35%, 75.26% and 94.29%, respectively, and the pig houses were mainly C. kinabaluensis, accounting for 69.44%. Through random sampling, PCR detection was performed on 190 full blood-sucking Culicoides gastric blood from 6 types of Culicoides. All 6 types of Culicoides had facultative blood-sucking phenomenon and sucked the blood of 4-6 kinds of animals at the same time. Among them, C. actoni and C. arakawai had the ability to suck the habit of human blood, accounted for 56.52% and 1.39% respectively. Therefore, this study preliminarily confirmed that the mitochondrial CO Ⅰ gene sequence of the species can be used to detect the source of gastric blood from the blood-sucking Culicoides. At the same time, it was found that all six Culicoides were facultative blood-sucking, with various blood-sucking habits and a wide range of blood-sucking objects.

Key words： blood-sucking midge gastric blood source CO Ⅰ gene; blood-sucking preference different livestock barn

Received: 12 March 2021

R384.5

Fund:Supported by the National Natural Science Foundation of China (No.81802040, No.31960102), and Innovation and Entrepreneurship Training Program for College Students of Zunyi Medical University (No.ZYDC2018003).

Corresponding Authors: Hou Xiao-hui,Email:hxh19801122@163.com

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LU Xue
	WANG Lin
	XU Yuan-yuan
	LIU Jiang-xin
	DAI Qing-lang
	HOU Xiao-hui

Cite this article:

LU Xue,WANG Lin,XU Yuan-yuan等. Molecular identification of gastric blood source of Culicoides in Ganshui Town, Qijiang, Chongqing, China[J]. Chinese Journal of Zoonoses, 2021, 37(12): 1064-1070.

URL:

http://www.rsghb.cn/EN/10.3969/j.issn.1002-2694.2021.00.155 OR http://www.rsghb.cn/EN/Y2021/V37/I12/1064

[1] Borkent A, Dominiak P.Catalog of the biting midges of the world (diptera: ceratopogonidae)[J]. Zootaxa, 2020, 4787(1): 377. DOI: 10.11646/zootaxa.4787.1.1
[2] 杨定, 李竹, 刘启飞,等.中国生物物种名录[M]. 北京:科学出版社, 2020:119-220.
[3] Fontaine A, Diouf I, Bakkali N, et al.Implication of haematophagous arthropod salivary proteins in host-vector interactions[J]. Parasit Vector, 2011, 4: 187. DOI: 10.1186/1756-3305-4-187
[4] Lehiy CJ, Drolet BS.The salivary secretome of the biting midge, Culicoides sonorensis[J]. PeerJ, 2014, 2(1): e426. DOI: 10.7717/peerj.426
[5] Russell CL, Heesom KJ, Arthur CJ, et al.Identification and isolation of cDNA clones encoding the abundant secreted proteins in the saliva proteome of Culicoides nubeculosus[J]. Insect Mol Biol, 2009, 18: 383-393. DOI: 10.1111/j.1365-2583.2009.00882.x
[6] Lehmann K, Werner D, Hoffmann B, et al.PCR identification of Culicoid biting midges (Diptera, Ceratopogonidae) of the Obsoletus complex including putative vectors of bluetongue and Schmallenberg viruses[J]. Parasit Vector, 2012, 5: 213. DOI: 10.1186/1756-3305-5-213
[7] 范娜, 程睿, 鲁晓晴, 等. 蠓虫及其传播的虫媒病毒[J]. 中国热带医学, 2018, 18(2): 182-187.
[8] 刘国平, 曹玉玺, 龙浩宇, 等. 湖南省新晃县和城步县吸血蠓的调查研究[J]. 中华卫生杀虫药械, 2016, 22(6): 586-588.
[9] 常琼琼, 段琛, 韩晓静, 等. 贵州省绥阳县吸血蠓类的调查研究[J]. 中国媒介生物学及控制杂志, 2017, 28(2): 138-140. DOI:10.11853/j.issn.1003-8280.2017.02.010
[10] England ME, Pearce-Kelly P, Brugman VA, et al.Culicoides species composition and molecular identification of host blood meals at two zoos in the UK[J]. Parasite Vector, 2020, 13:139. DOI:10.1186/s13071-020-04018-0
[11] 刘仰青, 陶卉英, 虞以新, 等. 吸血库蠓胃血血源分子鉴定及吸血习性多样性研究[J]. 中国媒介生物学及控制杂志, 2019, 30(4): 418-421. DOI:10.11853/j.issn.1003-8280.2019.04.014
[12] 岳巧云, 邱德义, 黄艺文, 等. 应用DNA 条形码技术鉴定未知双翅目蛹[J]. 中国国境卫生检疫杂志, 2011, 34(5): 343-347.
[13] Hebert PDN, Cywinska A, Ball SL, et al.Biological identifications through DNA barcodes[J]. Proc Biol Sci, 2003, 270(7): 313-321. DOI: 10.1098/rspb.2002.2218
[14] 虞以新, 刘金华, 刘国平,等. 中国蠓科昆虫[M]. 北京: 军事医学科学出版社, 2005:989-1036.
[15] 刘国平, 孙定炜, 范娜, 等. 海南省5市县禽畜厩舍吸血蠓调查[J].中国热带医学, 2020, 20(5): 413-416,428.
[16] 杨军, 张明, 王泽, 等. 云南河口口岸吸血蠓调查[J]. 中国国境卫生检疫杂志, 2018, 41(3): 170-172.
[17] 任清明, 刘国平, 杨军, 等. 辽宁省宽甸县吸血蠓种群调查研究[J]. 中华卫生杀虫药械, 2020, 26(5): 433-435.
[18] 任清明, 刘国平, 王治, 等. 东北3省7县(市)牛舍吸血蠓调查研究[J]. 中国媒介生物学及控制杂志,2021,32(1):5.
[19] Hebert PDN, Ratnasingham S, deWaard JR. Barcoding animal life: cytochrome coxidase subunit I divergences among closely related species[J]. Proc Biol Sci, 2003, 270(Suppl 1): 96-99. DOI: 10.1098/rsbl.2003.0025
[20] Tomazatos A, Jöst H, Schulze J, et al.Blood-meal analysis of Culicoides (Diptera: Ceratopogonidae) reveals a broad host range and new species records for Romania[J]. Parasit Vector, 2020, 13: 79. DOI: 10.1186/s13071-020-3938-1