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| Analysis of virulence genes and molecular typing of non-O1/O139 Vibrio cholerae in a link freshwater aquaculture in Henan Province |
| CHUI Hui-xia1, CHEN Lei2, WU Ling-ling1, JIA Song-shu1, HAN Zhi-wei1 |
1. Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China;
2. Kaifeng Center for Disease Control and Prevention,Kaifeng 475000, China |
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Abstract To investigate the distribution and molecular typing of virulence genes of non O1/O139 Vibrio cholerae in freshwater aquaculture in Henan. Fifty non O1/O139 Vibrio cholerae strains and 3 patient strains from freshwater aquaculture in Henan were sequenced, and their sequence typing (ST) was analyzed by PubMLST-Vc database. The evolutionary relationship was analyzed by using the minimum spanning tree diagram, and the distribution of virulence genes was obtained through VFDB database. The 53 strains of non-O1/O139 Vibrio cholerae from freshwater aquaculture and patients all have 8 kinds of virulence related factor genes with functions of adhesion, chemotaxis, anti phagocytosis, toxin and enzyme, and that didn’t carried the virulence related factor genes with functions of auxiliary colonization, toxin co regulation, secretion and the toxin genes of paracholesterone enterotoxin and other four toxin genes. The virulence genes of some virulence related factors or some strains were incomplete. Compared with the three strains from patients, the virulence factor gene carrying of the two strains was similar, except for the mshA gene, the podocyte wbuB gene, the wbfY gene, the rm1B gene, the heme receptor hutA gene and the type Ⅲ secretion system vscC2 and vcrD2 genes, Some strains carried the same virulence gene. The 53 strains of non-O1/O139 Vibrio cholerae belonged to 19 ST types. ST4 and ST5 were the dominant ST types. The strains from breeding and patients belonged to different ST types. The number of allelic locus variation differences of the 19 STs is 1-7. Among them, 17 STs of non-O1/O139 Vibrio cholerae strains from freshwater aquaculture had 1-7 allelic locus variation differences. Two STs of non-O1/O139 Vibrio cholerae strains from patients belonged to the same cluster as those of non-O1/O139 Vibrio cholerae strains from freshwater aquaculture, with 6 allelic locus variation differences from ST1. MLST typing of non-O1/O139 Vibrio cholerae strains in freshwater aquaculture in Henan is diversified, carrying a variety of virulence related factors. Some strains and patients’ strains carry the same virulence genes. Although they belong to different ST types, there are still food safety risks, which reminds the relevant departments to take measures to prevent and control.
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Received: 12 March 2021
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Corresponding Authors:
Chui Hui-xia,Email:156038777@qq.com
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2021, Vol. 37 
