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| Investigation on Rickettsia carried by rodents in Luxi County, Yunnan Province |
| YUAN Qing-hong1, YANG Li-gui2, TIAN Jie1, YANG Wei-hong1, ZHOU Ji-hua1, YANG Xiang-dong1, YU Bin-bin1, LUO De-rong2, YANG Jiao2 |
1. Yunnan Institute of Endemic Disease Control and Prevention/Yunnan Provincial Key Laboratory of Natural Focal Disease Control and Prevention, Dali 671000, China;
2. Luxi County Center for Disease Control and Prevention, Luxi 652400, China |
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Abstract To investigate the status of rodents infected with orientia tsutsugamushi, Anaplasma and Ehrlichia in Luxi County, Yunnan Province, to understand the preservation status and genetic characteristics of these pathogens in local nature. Using rat-cage and rattrap to catch rats in Luxi County. The captured animals were classified identified and their spleen was cut off, and the live mice were collected blood. To amplify the oriental groEL gene and specific 16S rRNA fragments of Anaplasma and Ehrlichia in mouse spleen by nest PCR. The DNA sequence of positive products was determined and the sequence alignment and phylogenetic analysis were carried out. Detection of oriental IgG antibody in rat serum by IFA. A total of 225 rodents of 10 species were caught in Luxi County. Among them, the dominant species Rattus flavipectus 83(36.89%), Eothenomys miletus 79(35.11%) and Apodemus draco 31(13.78%). Obtained 85 rat serum. The oriental groEL gene was detected by pcr from 5 samples, 2 from Rattus flavipectus and 3 from Eothenomys miletus, with a sequence similarity of 99.02%~100%. The phylogenetic analysis showed that strains of tsutsugamushi were located in the same branch as the strains of japan, thailand and Anhui, china.Of the 16S rRNA genes detected in 3 other rat samples, 1 was Ehrlichia gene from the Eothenomys miletus,2 were Bartonella and Wolbachia genes from Rattus norvegicus. Anaplasma, and the Ehrlichia gene sequence was 98.0%~100% homologous to the strains of United States, China and Brazil. Phylogenetic analysis showed that it was most related to Erythrin in the United States。7 positive samples of tsutsugamushi IgG antibody were detected (8.24%). There are natural foci of scrub typhus in this area, with Ruttus flauipectus and Eothenomys as the main hosts, and a few rodents carry Ehrlichia, Bartonella or Wolbachia.
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Received: 13 January 2021
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2021, Vol. 37 
